Cell-free synthetic biology in the new era of enzyme engineering

Nan Jiang; Lianju Ma; Yuan Lu

doi:10.1016/j.cjche.2020.05.037

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Cell-free synthetic biology in the new era of enzyme engineering

Special Topic: Biocatalysis | Updated：2026-01-06

- Cell-free synthetic biology in the new era of enzyme engineering
- Cell-free synthetic biology in the new era of enzyme engineering
- 中国化学工程学报（英文版） 2020年28卷第11期页码：2810-2816
- Affiliations：
  
  1. College of Life Sciences, Shenyang Normal University,Shenyang,China,110034
  2. Key Lab of Industrial Biocatalysis, Ministry of Education, Department of Chemical Engineering, Tsinghua University,Beijing,China,100084
- Author bio：
- Funds：
- DOI：10.1016/j.cjche.2020.05.037
  中图分类号：
- 纸质出版：2020
- Accepted：
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Nan Jiang, Lianju Ma, Yuan Lu. Cell-free synthetic biology in the new era of enzyme engineering[J]. 中国化学工程学报（英文版）, 2020,28(11):2810-2816.

Nan Jiang, Lianju Ma, Yuan Lu. Cell-free synthetic biology in the new era of enzyme engineering[J]. Chinese Journal of Chemical Engineering, 2020, 28(11): 2810-2816.
Nan Jiang, Lianju Ma, Yuan Lu. Cell-free synthetic biology in the new era of enzyme engineering[J]. 中国化学工程学报（英文版）, 2020,28(11):2810-2816. DOI： 10.1016/j.cjche.2020.05.037.

Nan Jiang, Lianju Ma, Yuan Lu. Cell-free synthetic biology in the new era of enzyme engineering[J]. Chinese Journal of Chemical Engineering, 2020, 28(11): 2810-2816. DOI： 10.1016/j.cjche.2020.05.037.

摘要

Abstract

With the gradual rise of enzyme engineering

it has played an essential role in synthetic biology

medicine

and biomanufacturing. However

due to the limitation of the cell membrane

the complexity of cellular metabolism

the difficulty of controlling the reaction environment

and the toxicity of some metabolic products in traditional

in vivo

enzyme engineering

it is usually problematic to express functional enzymes and produce a high yield of synthesized compounds. Recently

cell-free synthetic biology methods for enzyme engineering have been proposed as alternative strategies. This cell-free method has no limitation of the cell membrane and no need to maintain cell viability

and each biosynthetic pathway is highly flexible. This property makes cell-free approaches suitable for the production of valuable products such as functional enzymes and chemicals that are difficult to synthesize. This article aims to discuss the latest advances in cell-free enzyme engineering

assess the trend of this developing topical filed

and analyze its prospects.

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