Microenvironmental engineering: An effective strategy for tailoring enzymatic activities

Yifei Zhang; Henry Hess

doi:10.1016/j.cjche.2020.04.010

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Microenvironmental engineering: An effective strategy for tailoring enzymatic activities

Reviews | Updated：2026-01-06

- Microenvironmental engineering: An effective strategy for tailoring enzymatic activities
- Microenvironmental engineering: An effective strategy for tailoring enzymatic activities
- 中国化学工程学报（英文版） 2020年28卷第8期页码：2028-2036
- Affiliations：
  
  Department of Biomedical Engineering, Columbia University, 351 L Engineering Terrace, 1210 Amsterdam Avenue,NY,New York,USA,10027
- Author bio：
- Funds：
- DOI：10.1016/j.cjche.2020.04.010
  中图分类号：
- 纸质出版：2020
- Accepted：
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Yifei Zhang, Henry Hess. Microenvironmental engineering: An effective strategy for tailoring enzymatic activities[J]. 中国化学工程学报（英文版）, 2020,28(8):2028-2036.

Yifei Zhang, Henry Hess. Microenvironmental engineering: An effective strategy for tailoring enzymatic activities[J]. Chinese Journal of Chemical Engineering, 2020, 28(8): 2028-2036.
Yifei Zhang, Henry Hess. Microenvironmental engineering: An effective strategy for tailoring enzymatic activities[J]. 中国化学工程学报（英文版）, 2020,28(8):2028-2036. DOI： 10.1016/j.cjche.2020.04.010.

Yifei Zhang, Henry Hess. Microenvironmental engineering: An effective strategy for tailoring enzymatic activities[J]. Chinese Journal of Chemical Engineering, 2020, 28(8): 2028-2036. DOI： 10.1016/j.cjche.2020.04.010.

摘要

Abstract

Rationally

engineering a favorable physicochemical microenvironment for enzymes has recently emerged as an effective strategy to improve their catalytic performance. In this review

we discuss four microenvironmental effects according to the mechanism of action: localizing and excluding reactants and regulators

regulating microenvironmental pH

creating a water-like microenvironment

and increasing the local temperature. These mechanisms are enzyme-independent and can in principle be used in combination to tailor enzyme behaviors

offering new approaches to enabling

enhancing

and regulating enzyme catalysis in diverse applications without the need for genetic engineering.

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