As a natural aromatic polymer

lignin has great potential but limited industrial application due to its complex chemical structure. Among strategies for lignin conversion

biodegradation has attracted promising interest recently in term of efficiency

selectivity and mild condition. In order to overcome the issues of poor stability and non-reusability of enzyme in the biodegradation of lignin

this work explored a protocol of immobilized laccase on magnetic nanoparticles (MNPs) with rough surfaces for enhanced lignin model compounds degradation. Scanning electron microscope with energy dispersive spectrometer (SEM-EDS)

flourier transformation infrared spectroscopy (FTIR) and thermal gravimetric analysis (TGA) were utilized to characterize the immobilization of laccase. The results showed a maximum activity recovery of 64.7% towards laccase when it was incubated with MNPs and glutaraldehyde (GA) with concentrations of 6 mg·ml

-1

and 7.5 mg·ml

-1

for 5 h

respectively. The immobilized laccase showed improved thermal stability and pH tolerance compared with free laccase

and remained more than 80% of its initial activity after 20 days of storage at 4 ℃. In addition

about 40% resid

ual activity of the laccase remained after 8 times cycles. Gas chromatography-mass spectrometry (GC-MS) was utilized to characterize the products of lignin model compound degradation and activation

and the efficiency of immobilized laccase was calculated to be 1-5 times that of free laccase. It was proposed that the synergistic effect between MNPs and laccase displays an important role in the enhancement of stability and activity in lignin model compound biodegradation.