Immobilization of laccase from <i>Myceliophthora thermophila</i> on functionalized silica nanoparticles: Optimization and application in lindane degradation

Jelena Bebić; Katarina Banjanac; Marija Ćorović; Ana Milivojević; Milica Simović; Aleksandar Marinković; Dejan Bezbradica

doi:10.1016/j.cjche.2019.12.025

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Immobilization of laccase from Myceliophthora thermophila on functionalized silica nanoparticles: Optimization and application in lindane degradation

Biotechnology and Bioengineering | Updated：2026-01-06

- Immobilization of laccase from Myceliophthora thermophila on functionalized silica nanoparticles: Optimization and application in lindane degradation
- Immobilization of laccase from Myceliophthora thermophila on functionalized silica nanoparticles: Optimization and application in lindane degradation
- 中国化学工程学报（英文版） 2020年28卷第4期页码：1136-1144
- Affiliations：
  
  1. Directorate of Measures and Precious Metals, Mike Alasa 14, Belgrade11000, Serbia
  2. Innovation Centre of Faculty of Technology and Metallurgy, University of Belgrade, Karnegijeva 4, Belgrade11000, Serbia
  3. Department of Biochemical Engineering and Biotechnology, Faculty of Technology and Metallurgy, University of Belgrade, Karnegijeva 4, Belgrade11000, Serbia
  4. Department of Organic Chemistry, Faculty of Technology and Metallurgy, University of Belgrade, Karnegijeva 4, Belgrade11000, Serbia
- Author bio：
- Funds：
- DOI：10.1016/j.cjche.2019.12.025
  中图分类号：
- 纸质出版：2020
- Accepted：
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Jelena Bebić, Katarina Banjanac, Marija Ćorović, 等. Immobilization of laccase from Myceliophthora thermophila on functionalized silica nanoparticles: Optimization and application in lindane degradation[J]. 中国化学工程学报（英文版）, 2020,28(4):1136-1144. DOI: 10.1016/j.cjche.2019.12.025.

on functionalized silica nanoparticles: Optimization and application in lindane degradation[J]. 中国化学工程学报, 2020, 28(4): 1136-1144.
Jelena Bebić, Katarina Banjanac, Marija Ćorović, 等. Immobilization of laccase from Myceliophthora thermophila on functionalized silica nanoparticles: Optimization and application in lindane degradation[J]. 中国化学工程学报（英文版）, 2020,28(4):1136-1144. DOI: 10.1016/j.cjche.2019.12.025. DOI：

on functionalized silica nanoparticles: Optimization and application in lindane degradation[J]. 中国化学工程学报, 2020, 28(4): 1136-1144. DOI： 10.1016/j.cjche.2019.12.025.

摘要

Abstract

This work is focused on immobilization of laccase from

Myceliophthora thermophila

expressed in Aspergillus oryzae (Novozym 51003

laccase) on amino modified fumed nano-silica (AFNS) and the possible use in bioremediation. Hereby

for the first time

factors affecting the immobilization of Novozym 51003

laccase on AFNS were investigated for defining the immobilization mechanism and optimizing the utilization of AFNS as support for laccase immobilization. The highest specific activity (13.1 IU·mg

-1

proteins) was achieved at offered 160 mg per g of AFNS and for the same offered protein concentration the highest activity immobilization yield

reaching 68.3% after the equilibrium time

at optimum pH 5.0

was obtained. Laccase immobilization occurs by adsorption as monolayer enzyme binding in 40 min

following pseudo-first-order kinetics. The possible use of obtained immobilized preparation was investigated in degradation of pesticide lindane. Within 24 h

lindane concentration was reduced to 56.8% of initial concentration and after seven repeated reuses it retained 70% of the original activity.

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Immobilization of laccase from Myceliophthora thermophila on functionalized silica nanoparticles: Optimization and application in lindane degradation

Immobilization of laccase from Myceliophthora thermophila on functionalized silica nanoparticles: Optimization and application in lindane degradation

DOI：10.1016/j.cjche.2019.12.025

摘要

Abstract

关键词

Keywords

references