Highly efficient synthesis of (R)-1,3-butanediol via anti-Prelog reduction of 4-hydroxy-2-butanone with absolute stereoselectivity by a newly isolated Pichia kudriavzevii

Han Zu; Hui Zhang; Anwen Fan; Jie Gu; Yao Nie; Pengjie Luo; Yan Xu

doi:10.1016/j.cjche.2019.12.015

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Highly efficient synthesis of (R)-1,3-butanediol via anti-Prelog reduction of 4-hydroxy-2-butanone with absolute stereoselectivity by a newly isolated Pichia kudriavzevii

Biotechnology and Bioengineering | Updated：2026-01-06

- Highly efficient synthesis of (R)-1,3-butanediol via anti-Prelog reduction of 4-hydroxy-2-butanone with absolute stereoselectivity by a newly isolated Pichia kudriavzevii
- Highly efficient synthesis of (R)-1,3-butanediol via anti-Prelog reduction of 4-hydroxy-2-butanone with absolute stereoselectivity by a newly isolated Pichia kudriavzevii
- 中国化学工程学报（英文版） 2020年28卷第8期页码：2160-2166
- Affiliations：
  
  1. School of Biotechnology and Key Laboratory of Industrial Biotechnology of Ministry of Education, Jiangnan University,Wuxi,China,214122
  2. China National Center for Food Safety Risk Assessment, NHC Key Laboratory of Food Safety Risk Assessment,Beijing,China,100022
  3. State Key Laboratory of Food Science and Technology, Jiangnan University,Wuxi,China,214122
- Author bio：
- Funds：
- DOI：10.1016/j.cjche.2019.12.015
  中图分类号：
- 纸质出版：2020
- Accepted：
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Han Zu, Hui Zhang, Anwen Fan, 等. Highly efficient synthesis of (R)-1,3-butanediol via anti-Prelog reduction of 4-hydroxy-2-butanone with absolute stereoselectivity by a newly isolated Pichia kudriavzevii[J]. 中国化学工程学报（英文版）, 2020,28(8):2160-2166. DOI: 10.1016/j.cjche.2019.12.015.

)-1,3-butanediol
Han Zu, Hui Zhang, Anwen Fan, 等. Highly efficient synthesis of (R)-1,3-butanediol via anti-Prelog reduction of 4-hydroxy-2-butanone with absolute stereoselectivity by a newly isolated Pichia kudriavzevii[J]. 中国化学工程学报（英文版）, 2020,28(8):2160-2166. DOI: 10.1016/j.cjche.2019.12.015. DOI：

)-1,3-butanediol DOI： 10.1016/j.cjche.2019.12.015.

摘要

Abstract

(

)-1

3-butanediol is an important pharmaceutical intermediate

and the synthesis of (

)-1

3-butanediol using green biological methods has recently been of interest for industrial application. Here

a novel strain QC-1 that efficiently transforms 4-hydroxy-2-butanone to (

)-1

3-butanediol was isolated from soil samples. Based on morphological

physiological

and biochemical tests and 5.8S-internal transcribed spacer sequencing

the strain was identified as

Pichia kudriavzevii

QC-1. The reaction conditions were optimized to 35 ℃

pH 8.0

rotation speed 200 rpm

and 6:5 mass ratio of glucose to 4-hydroxy-2-butanone. Evaluation of the effects of 4-hydroxy- 2-butanone concentrations on yield and cell survival rate showed that 85.60 g·L

-1

product accumulated

with an enantiomeric excess of more than 99%

when 30 g·L

-1

4-hydroxy-2-butanone was added at 0

and 30 h in a 3-L bioreactor. Thus

strain QC-1 showed excellent catalytic activity and stereoselectivity for the synthesis of (

)-1

3-butanediol from 4-hydroxy-2-butanone.

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