A novel aeration strategy in repeated-batch fermentation for efficient ethanol production from sweet sorghum juice

Niphaphat Phukoetphim; Naulchan Khongsay; Pattana Laopaiboon; Lakkana Laopaiboon

doi:10.1016/j.cjche.2018.11.010

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A novel aeration strategy in repeated-batch fermentation for efficient ethanol production from sweet sorghum juice

Biotechnology and Bioengineering | Updated：2026-01-06

- A novel aeration strategy in repeated-batch fermentation for efficient ethanol production from sweet sorghum juice
- A novel aeration strategy in repeated-batch fermentation for efficient ethanol production from sweet sorghum juice
- 中国化学工程学报（英文版） 2019年27卷第7期页码：1651-1658
- Affiliations：
  
  1. Department of Biotechnology, Faculty of Technology, Khon Kaen University, Khon Kaen,Thailand,40002
  2. Graduate School, Khon Kaen University, Khon Kaen,Thailand,40002
  3. Fermentation Research Center for Value Added Agricultural Products, Khon Kaen University, Khon Kaen,Thailand,40002
- Author bio：
- Funds：
- DOI：10.1016/j.cjche.2018.11.010
  中图分类号：
- 纸质出版：2019
- Accepted：
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Niphaphat Phukoetphim, Naulchan Khongsay, Pattana Laopaiboon, 等. A novel aeration strategy in repeated-batch fermentation for efficient ethanol production from sweet sorghum juice[J]. 中国化学工程学报（英文版）, 2019,27(7):1651-1658.

Niphaphat Phukoetphim, Naulchan Khongsay, Pattana Laopaiboon, Lakkana Laopaiboon. A novel aeration strategy in repeated-batch fermentation for efficient ethanol production from sweet sorghum juice[J]. Chinese Journal of Chemical Engineering, 2019, 27(7): 1651-1658.
Niphaphat Phukoetphim, Naulchan Khongsay, Pattana Laopaiboon, 等. A novel aeration strategy in repeated-batch fermentation for efficient ethanol production from sweet sorghum juice[J]. 中国化学工程学报（英文版）, 2019,27(7):1651-1658. DOI： 10.1016/j.cjche.2018.11.010.

Niphaphat Phukoetphim, Naulchan Khongsay, Pattana Laopaiboon, Lakkana Laopaiboon. A novel aeration strategy in repeated-batch fermentation for efficient ethanol production from sweet sorghum juice[J]. Chinese Journal of Chemical Engineering, 2019, 27(7): 1651-1658. DOI： 10.1016/j.cjche.2018.11.010.

摘要

Abstract

To improve the efficiency of ethanol production in a batch fermentation from sweet sorghum juice under a very high gravity (VHG) condition (~290 g/L of total sugar) by

Saccharomyces cerevisiae

NP01

repeatedbatch fermentation under an aerated condition (2.5 vvm for the first 4 h during every cycle) was done in a 5-L fermenter. The average ethanol concentration (

)

productivity (

) and yield (

p/s

) for five successive cycles were 112.31 g/L

1.55 g/L·h

-1

and 0.44

respectively with 80.97% sugar consumption. To complete sugar consumption

the total sugar of the juice was reduced to a high gravity (HG) level (~240 g/L). The results showed that yeast extract was not necessary for ethanol production

and aeration during every other cycle

i.e

alternating cycles

was sufficient to promote both yeast growth and ethanol production. The average

and

p/s

values for eight successive cycles with aeration during alternating cycles were 97.58 g/L

1.98 g/L·h and 0.41

respectively with 91.21% sugar consumption. The total fatty acids in the yeast cells under the aerated condition we

re~50% higher than without aeration

irrespective the initial sugar concentration

whereas the ergosterol contents under aeration condition were~29% to 49% higher than those without aeration.

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