Adsorption behaviors of avian immunoglobulins and purification of immunoglobulin Y from chicken serum with mixed-mode resins

Yingdi Luo; Qilei Zhang; Shanjing Yao; Dongqiang Lin

doi:10.1016/j.cjche.2018.05.011

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Adsorption behaviors of avian immunoglobulins and purification of immunoglobulin Y from chicken serum with mixed-mode resins

Separation Science and Engineering | Updated：2026-01-06

- Adsorption behaviors of avian immunoglobulins and purification of immunoglobulin Y from chicken serum with mixed-mode resins
- Adsorption behaviors of avian immunoglobulins and purification of immunoglobulin Y from chicken serum with mixed-mode resins
- 中国化学工程学报（英文版） 2019年27卷第3期页码：514-518
- Affiliations：
  
  Key Laboratory of Biomass Chemical Engineering of Ministry of Education, College of Chemical and Biological Engineering, Zhejiang University,Hangzhou,China,310027
- Author bio：
- Funds：
- DOI：10.1016/j.cjche.2018.05.011
  中图分类号：
- 纸质出版：2019
- Accepted：
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Yingdi Luo, Qilei Zhang, Shanjing Yao, 等. Adsorption behaviors of avian immunoglobulins and purification of immunoglobulin Y from chicken serum with mixed-mode resins[J]. 中国化学工程学报（英文版）, 2019,27(3):514-518.

Yingdi Luo, Qilei Zhang, Shanjing Yao, Dongqiang Lin. Adsorption behaviors of avian immunoglobulins and purification of immunoglobulin Y from chicken serum with mixed-mode resins[J]. Chin.J.Chem.Eng., 2019, 27(3): 514-518.
Yingdi Luo, Qilei Zhang, Shanjing Yao, 等. Adsorption behaviors of avian immunoglobulins and purification of immunoglobulin Y from chicken serum with mixed-mode resins[J]. 中国化学工程学报（英文版）, 2019,27(3):514-518. DOI： 10.1016/j.cjche.2018.05.011.

Yingdi Luo, Qilei Zhang, Shanjing Yao, Dongqiang Lin. Adsorption behaviors of avian immunoglobulins and purification of immunoglobulin Y from chicken serum with mixed-mode resins[J]. Chin.J.Chem.Eng., 2019, 27(3): 514-518. DOI： 10.1016/j.cjche.2018.05.011.

摘要

Abstract

The importance of immunoglobulin Y (IgY) as a specific antibody equivalent to mammalian immunoglobulin G (IgG) is well recognized. However

production of highly purified IgY is still difficult due to the lack of specific purification methods. In this study

adsorption behaviors of IgY on four mixed-mode resins with functional ligands of 4-mercatoethyl-pyridine (MEP)

2-mercapto-1-methyli-midazole (MMI)

5-aminobenzi-midazole (ABI) and tryptophan-5-aminobenzi-midazole (W-ABI) were evaluated. The results showed that high adsorption ratio were found at pH 6.0-7.0 with little adsorption under acidic conditions. The resin with ABI ligand was then used to separate IgY from immunized chicken serum. An efficient process with IgY purity of 95% and recovery of 90% was developed after optimization of loading and elution pH and injection volume. The biological activity of the purified IgY was fully maintained. These results indicated that mixed-mode chromatography with specially-designed ligands has great potential for the separation of IgY from crude feedstock.

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