Preparation and evaluation of mixed-mode resins with tryptophan analogues as functional ligands for human serum albumin separation

Qici Wu; Qilei Zhang; Shiwen Xu; Cheng ong Ge; Shanjing Yao; Dongqiang Lin

doi:10.1016/j.cjche.2016.12.010

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Preparation and evaluation of mixed-mode resins with tryptophan analogues as functional ligands for human serum albumin separation

Separation Science and Engineering | Updated：2026-01-06

- Preparation and evaluation of mixed-mode resins with tryptophan analogues as functional ligands for human serum albumin separation
- Preparation and evaluation of mixed-mode resins with tryptophan analogues as functional ligands for human serum albumin separation
- 中国化学工程学报（英文版） 2017年25卷第7期页码：898-905
- Affiliations：
  
  Key Laboratory of Biomass Chemical Engineering of Ministry of Education, College of Chemical and Biological Engineering, Zhejiang University,Hangzhou,China,310027
- Author bio：
- Funds：
- DOI：10.1016/j.cjche.2016.12.010
  中图分类号：
- 纸质出版：2017
- Accepted：
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Qici Wu, Qilei Zhang, Shiwen Xu, 等. Preparation and evaluation of mixed-mode resins with tryptophan analogues as functional ligands for human serum albumin separation[J]. 中国化学工程学报（英文版）, 2017,25(7):898-905.

Qici Wu, Qilei Zhang, Shiwen Xu, Cheng ong Ge, Shanjing Yao, Dongqiang Lin. Preparation and evaluation of mixed-mode resins with tryptophan analogues as functional ligands for human serum albumin separation[J]. , 2017, 25(7): 898-905.
Qici Wu, Qilei Zhang, Shiwen Xu, 等. Preparation and evaluation of mixed-mode resins with tryptophan analogues as functional ligands for human serum albumin separation[J]. 中国化学工程学报（英文版）, 2017,25(7):898-905. DOI： 10.1016/j.cjche.2016.12.010.

Qici Wu, Qilei Zhang, Shiwen Xu, Cheng ong Ge, Shanjing Yao, Dongqiang Lin. Preparation and evaluation of mixed-mode resins with tryptophan analogues as functional ligands for human serum albumin separation[J]. , 2017, 25(7): 898-905. DOI： 10.1016/j.cjche.2016.12.010.

摘要

Abstract

Five tryptophan analogues with a hydrophobic indole ring and an amino group on each molecule were used as functional ligands of mixed-mode resins for human serum albumin (HSA) purification. Their adsorption performance was evaluated and the effects of pH and salt addition on HSA adsorption were studied. The resins prepared showed typical pH-dependent adsorption and the highest adsorption capacity and affinity were found at pH 5.0 for all the resins tested. The saturated adsorption capacity was 138.02 mg·g

-1

with the tryptaminefunctionalized resin

which significantly decreased at pH below 4.0 due to electrostatic repulsion between ligands and HSA. Moreover

the addition of NaCl or (NH

)

in media reduced HSA adsorption capacity

although the two salts showed different affecting profiles. The tryptamine-functionalized resin showed the best salt-tolerant performance

and its high adsorption capacity was maintained under high salt concentrations. In addition

the five resins prepared showed good adsorption selectivity for recombinant HSA from

Pichia pastoris

broth. Molecular docking results between tryptamine

and HSA indicated that tryptamine was favorable to bind on Site Ⅱ (indole-binding site) of HSA.

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