Immobilization of Papain in Biosilica Matrix and Its Catalytic Property

周丽亚; 王翠; 姜艳军; 高静

doi:10.1016/S1004-9541(13)60528-5

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Immobilization of Papain in Biosilica Matrix and Its Catalytic Property

生物技术与生物工程 | Updated：2026-01-06

- Immobilization of Papain in Biosilica Matrix and Its Catalytic Property
- Immobilization of Papain in Biosilica Matrix and Its Catalytic Property
- 中国化学工程学报（英文版） 2013年第6期页码：670-6751004-954160528-5
- Affiliations：
  
  1. School of Chemical Engineering and Technology, Hebei University of Technology,Tianjin,China,300130
  2. 2 National Key Laboratory of Biochemical Engineering, Institute of Process Engineering, Chinese Academy of Sciences,Beijing,China,100190
- Author bio：
- Funds：
  
  Supported by the National Natural Science Foundation of China (21006020, 21276060, 21276062), the Natural Science Foundation of Hebei Province (B2010000035, B2011202095), the Science and Technology Research Key Project of Higher School in Hebei Province (ZD2010118)
- DOI：10.1016/S1004-9541(13)60528-5
  中图分类号：
- 纸质出版：2013
- Accepted：
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周丽亚, 王翠, 姜艳军, 等. Immobilization of Papain in Biosilica Matrix and Its Catalytic Property[J]. 中国化学工程学报（英文版）, 2013,(6):670-6751004-954160528-5.

ZHOU Liya, WANG Cui, JIANG Yanjun, et al. Immobilization of Papain in Biosilica Matrix and Its Catalytic Property[J]. Chinese Journal of Chemical Engineering, 2013, (6): 670-6751004-954160528-5.
周丽亚, 王翠, 姜艳军, 等. Immobilization of Papain in Biosilica Matrix and Its Catalytic Property[J]. 中国化学工程学报（英文版）, 2013,(6):670-6751004-954160528-5. DOI： 10.1016/S1004-9541(13)60528-5.

ZHOU Liya, WANG Cui, JIANG Yanjun, et al. Immobilization of Papain in Biosilica Matrix and Its Catalytic Property[J]. Chinese Journal of Chemical Engineering, 2013, (6): 670-6751004-954160528-5. DOI： 10.1016/S1004-9541(13)60528-5.

摘要

Abstract

A novel method was developed for papain immobilization through a biomimetic silicification process induced by papain. By incubating papain in a silica precursor solution

the papain-silica composite formed rapidly and papain was encapsulated. The encapsulation efficiency and the recovery activity were 82.60% and 83.09%

respectively. Compared with enzymes and biomolecules immobilized in biosilica matrix in the presence of additional silica-precipitating species

this papain encapsulation process

a biomimetic approach

realized high encapsulation efficiency by its autosilification activity under mild conditions (near-neutral pH and ambient temperature). Furthermore

the encapsulated papain exhibits enhanced thermal

recycling and storage stabilities. Kinetic analysis showed that the biomimetic silica matrix did not significantly hinder the mass transport of substrate or the release of product.

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