Optimization for Production of Intracellular Polysaccharide from <i>Cordyceps ophioglossoides</i> L2 in Submerged Culture and Its Antioxidant Activities in <i>vitro</i>

许勤勤; 刘振华; 孙一晟; 丁忠杰; 吕龙贤; 李永泉

doi:10.1016/S1004-9541(12)60391-7

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Optimization for Production of Intracellular Polysaccharide from Cordyceps ophioglossoides L2 in Submerged Culture and Its Antioxidant Activities in vitro

BIOTECHNOLOGY AND BIOENGINEERING | Updated：2026-01-06

- Optimization for Production of Intracellular Polysaccharide from Cordyceps ophioglossoides L2 in Submerged Culture and Its Antioxidant Activities in vitro
- Optimization for Production of Intracellular Polysaccharide from Cordyceps ophioglossoides L2 in Submerged Culture and Its Antioxidant Activities in vitro
- 中国化学工程学报（英文版） 2012年20卷第2期页码：294-301
- Affiliations：
  
  College of Life Sciences, Zhejiang University,Hangzhou,China,310058
- Author bio：
- Funds：
  
  Supported by the National High Technology Research and Development Program of China (2007AA021506);the Natural Science Foundation of Zhejiang Province (R207609);the Research Project of Science and Technology of Zhejiang Province,China (2005C23027)
- DOI：10.1016/S1004-9541(12)60391-7
  中图分类号：
- 纸质出版：2012
- Accepted：
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许勤勤, 刘振华, 孙一晟, 等. Optimization for Production of Intracellular Polysaccharide from Cordyceps ophioglossoides L2 in Submerged Culture and Its Antioxidant Activities in vitro[J]. 中国化学工程学报（英文版）, 2012,20(2):294-301. DOI: 10.1016/S1004-9541(12)60391-7.

L2 in Submerged Culture and Its Antioxidant Activities in
许勤勤, 刘振华, 孙一晟, 等. Optimization for Production of Intracellular Polysaccharide from Cordyceps ophioglossoides L2 in Submerged Culture and Its Antioxidant Activities in vitro[J]. 中国化学工程学报（英文版）, 2012,20(2):294-301. DOI: 10.1016/S1004-9541(12)60391-7. DOI：

L2 in Submerged Culture and Its Antioxidant Activities in DOI： 10.1016/S1004-9541(12)60391-7.

摘要

Abstract

Cordyceps ophioglossoides

is a valuable traditional medicinal material.We have found that intracellular polysaccharide(IPS) is the major biologically active ingredient in

Cordyceps ophioglossoides

.This study is the first time to optimize the yield of IPS from

Cordyceps ophioglossoides

.The optimal medium for IPS production consists of glucose 54.50 g·L

-1

yeast powder 25.50 g·L

-1

NaH

0.4 g·L

-1

and K

HPO

0.4 g·L

-1

.The suggested culture conditions are 24 ℃

initial pH 4.5 with a rotary speed of 120 r·min

-1

for 168 h.The yield of IPS is 737.93 mg·L

-1

which is 50% higher than the yield under the conditions prior to optimization.The anti-oxidative activities of IPS in

Cordyceps ophioglossoides

L2 are also characterized using various in vitro assay.The anti-oxidative activity may explain the reason why IPS from

Cordyceps ophioglossoides

can be used to fight against neurodegenerative dis-eases and menopausal symptoms.

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Optimization for Production of Intracellular Polysaccharide from Cordyceps ophioglossoides L2 in Submerged Culture and Its Antioxidant Activities in vitro

Optimization for Production of Intracellular Polysaccharide from Cordyceps ophioglossoides L2 in Submerged Culture and Its Antioxidant Activities in vitro

DOI：10.1016/S1004-9541(12)60391-7

摘要

Abstract

关键词

Keywords

references