Multiple Strategies for Metabolic Engineering of Escherichia coli for Efficient Production of Coenzyme Q<sub>10</sub>

黄明涛; 王玥; 刘建忠; 毛宗万

doi:10.1016/S1004-9541(11)60171-7

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Multiple Strategies for Metabolic Engineering of Escherichia coli for Efficient Production of Coenzyme Q₁₀

Updated：2026-01-06

- Multiple Strategies for Metabolic Engineering of Escherichia coli for Efficient Production of Coenzyme Q₁₀
- Multiple Strategies for Metabolic Engineering of Escherichia coli for Efficient Production of Coenzyme Q₁₀
- 中国化学工程学报（英文版） 2011年19卷第2期页码：316-326
- Affiliations：
  
  1. The Key Laboratory of Gene Engineering of Ministry of Education, State Key Laboratory of Biocontrol, Sun Yat-Sen University,Guangzhou,China,510275
  2. MOE Key Laboratory of Bioinorganic and Synthetic Chemistry, Sun Yat-Sen University,Guangzhou,China,510275
- Author bio：
- Funds：
  
  Supported by the National Natural Science Foundation of China(30970089,200876181,20831006);the Natural Science Foundation of Guangdong Province (9351027501000003);the Project of Science and Technology of Guangdong Province(2007A010900001)
- DOI：10.1016/S1004-9541(11)60171-7
  中图分类号：
- 纸质出版：2011
- Accepted：
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黄明涛, 王玥, 刘建忠, 等. Multiple Strategies for Metabolic Engineering of Escherichia coli for Efficient Production of Coenzyme Q₁₀[J]. 中国化学工程学报（英文版）, 2011,19(2):316-326. DOI: 10.1016/S1004-9541(11)60171-7.

[J]. , 2011, 19(2): 316-326.
黄明涛, 王玥, 刘建忠, 等. Multiple Strategies for Metabolic Engineering of Escherichia coli for Efficient Production of Coenzyme Q₁₀[J]. 中国化学工程学报（英文版）, 2011,19(2):316-326. DOI: 10.1016/S1004-9541(11)60171-7. DOI：

[J]. , 2011, 19(2): 316-326. DOI： 10.1016/S1004-9541(11)60171-7.

摘要

Abstract

Escherichia coli

BW25113 was metabolically engineered for CoQ

production by replacing

ispB

with ddsA from Gluconobacter suboxydans.Effects of precursor balance and reduced nicotinamide-adenine dinucleotide phosphate (NADPH) availability on CoQ

production in

.coli were investigated.The knockout of

pykFA

along with

pck

overexpression could maintain a balance between glyceraldehyde 3-phosphate and pyruvate

increasing CoQ

production.Replacement of native NAD-dependent

gapA

with NADP-dependent

gapC

from Clostridium acetobutylicum

together with the overexpression of

gapC

could increase NADPH availability and then enhanced CoQ

production.Three effects

overexpressions of various genes in CoQ biosynthesis and central metabolism

different vectors and culture conditions on CoQ

production in

E.coli

were all investigated.The investigation of different vectors indicated that low copy number vector may be more beneficial for CoQ

production in

E.coli

.The recombinant

E.coli

(△

ispB

ddsA

△

pykFA

and △

gapA

gapC

)

harboring the two plasmids encoding pck

dxs

idi and ubiCA genes under the control of P

on pQE30

ispA

ddsA

from

Gluconobacter suboxydans

and

gapC

from Clostridium acetobutylicum under the control of PBAD on pBAD33

could produce CoQ

up to

3.24 mg·g

-1

dry cell mass simply by changing medium from M9YG to SOB with phosphate salt and initial culture pH from 7.0 to 5.5.The yield is unprecedented and 1.33 times of the highest production so far in E.coli.

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Multiple Strategies for Metabolic Engineering of Escherichia coli for Efficient Production of Coenzyme Q10

Multiple Strategies for Metabolic Engineering of Escherichia coli for Efficient Production of Coenzyme Q10

DOI：10.1016/S1004-9541(11)60171-7

摘要

Abstract

关键词

Keywords

references

Multiple Strategies for Metabolic Engineering of Escherichia coli for Efficient Production of Coenzyme Q₁₀

Multiple Strategies for Metabolic Engineering of Escherichia coli for Efficient Production of Coenzyme Q₁₀