Isolation of Lysozyme from Chicken Egg White Using Polyacrylamide-based Cation-exchange Cryogel

晏禄丁; 沈绍传; 贠军贤; 姚克俭

doi:10.1016/S1004-9541(11)60068-2

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Isolation of Lysozyme from Chicken Egg White Using Polyacrylamide-based Cation-exchange Cryogel

Updated：2026-01-06

- Isolation of Lysozyme from Chicken Egg White Using Polyacrylamide-based Cation-exchange Cryogel
- Isolation of Lysozyme from Chicken Egg White Using Polyacrylamide-based Cation-exchange Cryogel
- 中国化学工程学报（英文版） 2011年19卷第5期页码：876-880
- Affiliations：
  
  State Key Laboratory Breeding Base of Green Chemistry Synthesis Technology, College of Chemical Engineering and Materials Science, Zhejiang University of Technology,Hangzhou,China,310032
- Author bio：
- Funds：
  
  Supported by the National Natural Science Foundation of China (21036005, 20876145);the Science and Technology Cooperation Project between China-Europe Country’s Governments from the Ministry of Science and Technology of China (1017);the Natural Science Foundation of Zhejiang Provincial (Y4080326)
- DOI：10.1016/S1004-9541(11)60068-2
  中图分类号：
- 纸质出版：2011
- Accepted：
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晏禄丁, 沈绍传, 贠军贤, 等. Isolation of Lysozyme from Chicken Egg White Using Polyacrylamide-based Cation-exchange Cryogel[J]. 中国化学工程学报（英文版）, 2011,19(5):876-880.

YAN Luding, SHEN Shaochuan, YUN Junxian, YAO Kejian. Isolation of Lysozyme from Chicken Egg White Using Polyacrylamide-based Cation-exchange Cryogel[J]. , 2011, 19(5): 876-880.
晏禄丁, 沈绍传, 贠军贤, 等. Isolation of Lysozyme from Chicken Egg White Using Polyacrylamide-based Cation-exchange Cryogel[J]. 中国化学工程学报（英文版）, 2011,19(5):876-880. DOI： 10.1016/S1004-9541(11)60068-2.

YAN Luding, SHEN Shaochuan, YUN Junxian, YAO Kejian. Isolation of Lysozyme from Chicken Egg White Using Polyacrylamide-based Cation-exchange Cryogel[J]. , 2011, 19(5): 876-880. DOI： 10.1016/S1004-9541(11)60068-2.

摘要

Abstract

An effective cation-exchange chromatographic method for lysozyme isolation from chicken egg white is presented

using supermacroporous cryogel grafted with sulfo functional groups. The chromatographic processes were carried out by one-step and sequential elution

respectively. Sodium phosphate buffer (pH 7.8) containing different concentrations of NaCl is used as elution agent. The corresponding breakthrough characteristics and elution behaviors in the cryogel bed were investigated and analyzed. Purity of lysozyme in the elution effluent was assayed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE). The maximum purity of the obtained lysozyme was about 96%

and the cryogel is demonstrated as a potential separation medium for purification of high-purity lysozyme from chicken egg white.

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