Salting-out Extraction of 2,3-Butanediol from Jerusalem artichoke-based Fermentation Broth

戴建英; 张媛丽; 修志龙

doi:10.1016/S1004-9541(11)60041-4

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Salting-out Extraction of 2,3-Butanediol from Jerusalem artichoke-based Fermentation Broth

Updated：2026-01-06

- Salting-out Extraction of 2,3-Butanediol from Jerusalem artichoke-based Fermentation Broth
- Salting-out Extraction of 2,3-Butanediol from Jerusalem artichoke-based Fermentation Broth
- 中国化学工程学报（英文版） 2011年19卷第4期页码：682-686
- Affiliations：
  
  School of Life Science and Biotechnology, Dalian University of Technology,Dalian,China,116024
- Author bio：
- Funds：
- DOI：10.1016/S1004-9541(11)60041-4
  中图分类号：
- 纸质出版：2011
- Accepted：
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戴建英, 张媛丽, 修志龙. Salting-out Extraction of 2,3-Butanediol from Jerusalem artichoke-based Fermentation Broth[J]. 中国化学工程学报（英文版）, 2011,19(4):682-686.

DAI Jianying, ZHANG Yuanli, XIU Zhilong. Salting-out Extraction of 2,3-Butanediol from Jerusalem artichoke-based Fermentation Broth [J]. , 2011, 19(4): 682-686.
戴建英, 张媛丽, 修志龙. Salting-out Extraction of 2,3-Butanediol from Jerusalem artichoke-based Fermentation Broth[J]. 中国化学工程学报（英文版）, 2011,19(4):682-686. DOI： 10.1016/S1004-9541(11)60041-4.

DAI Jianying, ZHANG Yuanli, XIU Zhilong. Salting-out Extraction of 2,3-Butanediol from Jerusalem artichoke-based Fermentation Broth [J]. , 2011, 19(4): 682-686. DOI： 10.1016/S1004-9541(11)60041-4.

摘要

Abstract

The removal of solid impurities and separation of target products from a fermentation broth is becoming more tedious with the utilization of lignocelluloses as source of substrate.2

3-Butanediol

an important chemical used widely is also a main product of sugar-based fermentation carried out by

Klebsiella pneumoniae

.In this study

we investigated the use of salting-out extraction(SOE) that employed a K

HPO

/ethanol system consisting of 21% ethanol and 17% K

HPO

(mass fraction) to separate 2

3-butanediol from the viscous Jerusalem artichoke-based fermentation broth.After SOE

about 98% of solid matters was removed

and the viscosity decreased from 72.5 mPa s in the original fermentation broth to 4.4 mPa s in the top phase.The partition coefficient and yield of 2

3-butanediol reached 13.4 and 99%

respectively

and 89% of soluble proteins was removed from the broth.The results showed that SOE is an efficient way for isolating 2

3-BD from a highly viscous fermentation broth by removing much of the solid matters within the broth.

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