Purification and Characterization of a Nonylphenol(NP)-degrading Enzyme from <i>Bacillus cereus</i>.Frankland

杨革; 张营; 白艳芬

doi:10.1016/S1004-9541(11)60035-9

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Purification and Characterization of a Nonylphenol(NP)-degrading Enzyme from Bacillus cereus.Frankland

Updated：2026-01-06

- Purification and Characterization of a Nonylphenol(NP)-degrading Enzyme from Bacillus cereus.Frankland
- Purification and Characterization of a Nonylphenol(NP)-degrading Enzyme from Bacillus cereus.Frankland
- 中国化学工程学报（英文版） 2011年19卷第4期页码：644-648
- Affiliations：
  
  1. College of Textiles Tianjin Polytechnic University,Tianjin,China,300160
  2. Key Lab of Biogeology and Environmental Geology of Ministry of Education, China University of Geosciences,Wuhan,China,430074
- Author bio：
- Funds：
- DOI：10.1016/S1004-9541(11)60035-9
  中图分类号：
- 纸质出版：2011
- Accepted：
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杨革, 张营, 白艳芬. Purification and Characterization of a Nonylphenol(NP)-degrading Enzyme from Bacillus cereus.Frankland[J]. 中国化学工程学报（英文版）, 2011,19(4):644-648. DOI: 10.1016/S1004-9541(11)60035-9.

.Frankland [J]. , 2011, 19(4): 644-648.
杨革, 张营, 白艳芬. Purification and Characterization of a Nonylphenol(NP)-degrading Enzyme from Bacillus cereus.Frankland[J]. 中国化学工程学报（英文版）, 2011,19(4):644-648. DOI: 10.1016/S1004-9541(11)60035-9. DOI：

.Frankland [J]. , 2011, 19(4): 644-648. DOI： 10.1016/S1004-9541(11)60035-9.

摘要

Abstract

AbstractAn extracellular NP-degrading enzyme secreted by

Bacillus cereus

.Frankland was purified to homogeneity by a combination of ammonium sulfate precipitation

Phenyl-Sepharose hydrophobic-interaction chromatography and DEAE anion-exchange chromatography.On SDS(sodium dodecyl sulfate)-polyacrylamide gel electrophoresis analysis

the purified enzyme showed a relative molecular mass of 58.3 kDa.The depolymerzation of subunits was accompanied with the loss of NP-degrading enzyme activity

and removing denaturing factors by dialysis could restore the dimer structure and enzymatic activity.The enzyme had an isoelectric point of 5.5 and an optimal temperature of 60℃

and was the most active at pH 6.0.The enzymatic activity was stable at pH 4-8 and inhibited by Cu

.Ten

-terminal amino acids were determined to be ASVNSIKIGY

demonstrating that the purified enzyme was a novel one.The hydrolysis pattern of the purified enzyme indicated that the NP-degrading enzyme was an endo NP-degrading enzyme.The extraordinary thermo-stability provided the enzyme with a good prospect to be used as a new tool for clean-production process for textile industry.

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Purification and Characterization of a Nonylphenol(NP)-degrading Enzyme from Bacillus cereus.Frankland

Purification and Characterization of a Nonylphenol(NP)-degrading Enzyme from Bacillus cereus.Frankland

DOI：10.1016/S1004-9541(11)60035-9

摘要

Abstract

关键词

Keywords

references