Identification and Mutagenesis of a New Isolated Strain <i>Bacillus</i> sp. B26 for Producing(<i>R</i>)-α-Hydroxyphenylacetic Acid

陈秉梅; 许小平; 侯志国; 李忠琴; 阮文兵

doi:10.1016/S1004-9541(11)60034-7

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Identification and Mutagenesis of a New Isolated Strain Bacillus sp. B26 for Producing(R)-α-Hydroxyphenylacetic Acid

Updated：2026-01-06

- Identification and Mutagenesis of a New Isolated Strain Bacillus sp. B26 for Producing(R)-α-Hydroxyphenylacetic Acid
- Identification and Mutagenesis of a New Isolated Strain Bacillus sp. B26 for Producing(R)-α-Hydroxyphenylacetic Acid
- 中国化学工程学报（英文版） 2011年19卷第4期页码：636-643
- Affiliations：
  
  1. College of Chemistry and Chemical Engineering, Fuzhou University,Fuzhou,China,350108
  2. College of Fisheries, Jimei University,Xiamen,China,361021
- Author bio：
- Funds：
  
  Supported by the Specialized Research Fund for the Doctoral Program of Higher Education of China (20103514110002);the Science and Technology Key Project of Fujian Province (2009N0046)
- DOI：10.1016/S1004-9541(11)60034-7
  中图分类号：
- 纸质出版：2011
- Accepted：
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陈秉梅, 许小平, 侯志国, 等. Identification and Mutagenesis of a New Isolated Strain Bacillus sp. B26 for Producing(R)-α-Hydroxyphenylacetic Acid[J]. 中国化学工程学报（英文版）, 2011,19(4):636-643. DOI: 10.1016/S1004-9541(11)60034-7.

sp. B26 for Producing(
陈秉梅, 许小平, 侯志国, 等. Identification and Mutagenesis of a New Isolated Strain Bacillus sp. B26 for Producing(R)-α-Hydroxyphenylacetic Acid[J]. 中国化学工程学报（英文版）, 2011,19(4):636-643. DOI: 10.1016/S1004-9541(11)60034-7. DOI：

sp. B26 for Producing( DOI： 10.1016/S1004-9541(11)60034-7.

摘要

Abstract

A bacterium strain B26 capable of producing(

)-α-hydroxyphenylacetic acid [(

)-HPA

]

(yield

47.5%;enantiomeric excess

99.1%) from phenylglyoxylic acid(PGA) with high optical purity was isolated and identified as

Bacillus

sp.B26 by 16S rDNA(ribosomal DNA) sequencing.Phylogenic analysis showed that the strain was most similar to

Bacillus

sp.enrichment culture clone SYW5(FJ601635.1) and

Bacillus

cereus strain FM-4(EU794727.1).Efforts were made to further improve HPA-production and PGA-tolerance by UV irradiation and UV-LiCl cooperative mutagenesis.Among viable mutants

.sp.UV-38 and B.sp.ULi-11 exhibited better productivities than the wild type.Comparisons of HPA production and time course among wild strain and two mutants showed that

.sp.ULi-11 was more competent than

.sp.UV-38.HPA production was increased by 39.1% with B.sp.ULi-11(yield

65.4%) compared to that with

.sp.B26(yield

47.0%) when cultured in fermentation broth(pH 7.2) at 32℃ with an agitation speed of 180 r·min

-1

and PGA final concentration of 15 mmol·L

-1

for 25 h.

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Identification and Mutagenesis of a New Isolated Strain Bacillus sp. B26 for Producing(R)-α-Hydroxyphenylacetic Acid

Identification and Mutagenesis of a New Isolated Strain Bacillus sp. B26 for Producing(R)-α-Hydroxyphenylacetic Acid

DOI：10.1016/S1004-9541(11)60034-7

摘要

Abstract

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Keywords

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