Deactivation Kinetics of Nitrile Hydratase in Free Resting Cells

孙旭东; 于慧敏; 沈忠耀

doi:10.1016/S1004-9541(08)60282-7

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Deactivation Kinetics of Nitrile Hydratase in Free Resting Cells

Updated：2026-01-06

- Deactivation Kinetics of Nitrile Hydratase in Free Resting Cells
- Deactivation Kinetics of Nitrile Hydratase in Free Resting Cells
- 中国化学工程学报（英文版） 2009年17卷第5期页码：822-828
- Affiliations：
  
  Department of Chemical Engineering, Tsinghua University,Beijing,China,100084
- Author bio：
- Funds：
  
  Supported by the Foundation for the Authors of National Excellent Doctoral Dissertation of China (200345);the National High Technology Research and Development Program of China (2007AA02Z201);the National Basic Research Program of China (2007CB714304)
- DOI：10.1016/S1004-9541(08)60282-7
  中图分类号：
- 纸质出版：2009
- Accepted：
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孙旭东, 于慧敏, 沈忠耀. Deactivation Kinetics of Nitrile Hydratase in Free Resting Cells[J]. 中国化学工程学报（英文版）, 2009,17(5):822-828.

SUN Xudong, YU Huimin, SHEN Zhongyao. Deactivation Kinetics of Nitrile Hydratase in Free Resting Cells[J]. , 2009, 17(5): 822-828.
孙旭东, 于慧敏, 沈忠耀. Deactivation Kinetics of Nitrile Hydratase in Free Resting Cells[J]. 中国化学工程学报（英文版）, 2009,17(5):822-828. DOI： 10.1016/S1004-9541(08)60282-7.

SUN Xudong, YU Huimin, SHEN Zhongyao. Deactivation Kinetics of Nitrile Hydratase in Free Resting Cells[J]. , 2009, 17(5): 822-828. DOI： 10.1016/S1004-9541(08)60282-7.

摘要

Abstract

Nitrile hydratase (NHase) is an important industrial enzyme used for acrylamide production from acrylonitrile.The deactivation kinetics of NHases in free resting cells of

Rhodococcus

sp.was presented based on a bi-steady state assumption.Effects of hydration temperature

product concentration and substrate concentration on NHase deactivation were investigated experimentally and correlated with a first order deactivation kinetics.The results showed that the hydration temperature and product concentration were major factors governing the deactivation of NHases under substrate-feeding conditions.When acrylamide concentration was higher than 250 g·L

-1

the deactivation of NHases became serious and the bi-steady state assumption was not applicable.When the hydration temperature was controlled at a relatively higher level such as 28℃

the total deactivation rate constant was about 2.8-fold of that at 20℃.

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