Resolution of Ibuprofen Ester by Catalytic Antibodies in Water-miscible Organic-solvents

杨根生; 应黎; 欧志敏; 姚善泾

doi:10.1016/S1004-9541(08)60238-4

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Resolution of Ibuprofen Ester by Catalytic Antibodies in Water-miscible Organic-solvents

Updated：2026-01-06

- Resolution of Ibuprofen Ester by Catalytic Antibodies in Water-miscible Organic-solvents
- Resolution of Ibuprofen Ester by Catalytic Antibodies in Water-miscible Organic-solvents
- 中国化学工程学报（英文版） 2009年17卷第3期页码：506-512
- Affiliations：
  
  1. Department of Chemical and Biochemical Engineering, Zhejiang University,Hangzhou,China,310027
  2. College of Pharmaceutical Sciences, Zhejiang University of Technology,Hangzhou,China,310032
- Author bio：
- Funds：
- DOI：10.1016/S1004-9541(08)60238-4
  中图分类号：
- 纸质出版：2009
- Accepted：
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杨根生, 应黎, 欧志敏, 等. Resolution of Ibuprofen Ester by Catalytic Antibodies in Water-miscible Organic-solvents[J]. 中国化学工程学报（英文版）, 2009,17(3):506-512.

YANG Gensheng, YING Li, OU Zhimin, YAO Shanjing. Resolution of Ibuprofen Ester by Catalytic Antibodies in Water-miscible Organic-solvents[J]. , 2009, 17(3): 506-512.
杨根生, 应黎, 欧志敏, 等. Resolution of Ibuprofen Ester by Catalytic Antibodies in Water-miscible Organic-solvents[J]. 中国化学工程学报（英文版）, 2009,17(3):506-512. DOI： 10.1016/S1004-9541(08)60238-4.

YANG Gensheng, YING Li, OU Zhimin, YAO Shanjing. Resolution of Ibuprofen Ester by Catalytic Antibodies in Water-miscible Organic-solvents[J]. , 2009, 17(3): 506-512. DOI： 10.1016/S1004-9541(08)60238-4.

摘要

Abstract

The asymmetric hydrolysis of racemic ibuprofen ester is one of the most important methods for chiral separation of ibuprofen. In this work

a catalytic antibody that accelerates the rate of enantioselective hydrolysis of ibuprofen methyl ester was obtained against an immunogen consisting of tetrahedral phosphonate hapten attached to bovine serum albumin (BSA). The catalytic activity of the catalytic antibody in the water-miscible organic-solvent system composed of a buffer solution and

-dimethylformamide (DMF) was studied. With 6% DMF in the buffer solution (containing catalytic antibody 0.25 μmol

0.2 mol·L

-1

phosphate buffer

pH 8) at 37℃ for 10 h

a good conversion (48.7%) and high enantiomeric excess (99%) could be reached. The kinetic analysis of the catalytic antibody-catalyzed reaction showed that the hydrolysis in the water-miscible organic-solvent system with DMF in buffer solution followed the Michaelis-Menten kinetics. The catalytic efficiency (

cat

) was enhanced to 151.91 L·mmol

-1

·min

-1

twice as large as that for the buffer solution only.

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