Isolation of <i>Cordyceps ophioglossoides</i> L2 from Fruit Body and Optimization of Fermentation Conditions for Its Mycelial Growth

许勤勤; 吕龙贤; 陈少云; 郑静; 郑高利; 李永泉

doi:10.1016/S1004-9541(08)60206-2

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Isolation of Cordyceps ophioglossoides L2 from Fruit Body and Optimization of Fermentation Conditions for Its Mycelial Growth

Updated：2026-01-06

- Isolation of Cordyceps ophioglossoides L2 from Fruit Body and Optimization of Fermentation Conditions for Its Mycelial Growth
- Isolation of Cordyceps ophioglossoides L2 from Fruit Body and Optimization of Fermentation Conditions for Its Mycelial Growth
- 中国化学工程学报（英文版） 2009年17卷第2期页码：278-285
- Affiliations：
  
  1. College of Life Sciences, Zhejiang University,Hangzhou,China,310058
  2. Institute of Materia Medica, Zhejiang Academy of Medical Sciences,Hangzhou,China,310013
- Author bio：
- Funds：
  
  Supported by the Research Project of Science and Technology of Zhejiang Province,China (2005C23027);the National High Technology Research and Development Program of China (2007AA021506);the Natural Science Foundation of Zhejiang Province (R207609)
- DOI：10.1016/S1004-9541(08)60206-2
  中图分类号：
- 纸质出版：2009
- Accepted：
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许勤勤, 吕龙贤, 陈少云, 等. Isolation of Cordyceps ophioglossoides L2 from Fruit Body and Optimization of Fermentation Conditions for Its Mycelial Growth[J]. 中国化学工程学报（英文版）, 2009,17(2):278-285. DOI: 10.1016/S1004-9541(08)60206-2.

L2 from Fruit Body and Optimization of Fermentation Conditions for Its Mycelial Growth[J]. , 2009, 17(2): 278-285.
许勤勤, 吕龙贤, 陈少云, 等. Isolation of Cordyceps ophioglossoides L2 from Fruit Body and Optimization of Fermentation Conditions for Its Mycelial Growth[J]. 中国化学工程学报（英文版）, 2009,17(2):278-285. DOI: 10.1016/S1004-9541(08)60206-2. DOI：

L2 from Fruit Body and Optimization of Fermentation Conditions for Its Mycelial Growth[J]. , 2009, 17(2): 278-285. DOI： 10.1016/S1004-9541(08)60206-2.

摘要

Abstract

A strain isolated from the fruiting body of a fungus parasitized on

Elaphomyces was identified as Cordyceps ophioglossoides

based on the morphological characteristics and the analysis of ITS-5.8s rDNA sequence.The optimal medium composition(g·L

-1

)

containing sucrose 66.0

yeast powder 10.0

silkworm chrysalises digest 30.0

MgSO

·7H

O 0.4

and KH

0.4

was found using fractional factorial design and a central composite design

and the optimization of cultural conditions obtained a result of seed age 6 days

inoculum size 6%(by volume)

initial pH 5.6

temperature 24℃

shaking speed 160 r·min

-1

by one-factor-at-a-time method.The maximum biomass reached about 20.2 g·L

-1

after 90 hours culture under the optimal conditions.Elementary pharmacological activities showed that mycelia of

C.ophioglossoides

L2 from submerged culture promoted uterus growth in estrogendepleted mice.In the 15-litre scale-up fermentation

the mycelial biomass was around 19.1 g·L

-1

indicating a promising prospect for this biotechnology and the potency to develop its medical value.

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Isolation of Cordyceps ophioglossoides L2 from Fruit Body and Optimization of Fermentation Conditions for Its Mycelial Growth

Isolation of Cordyceps ophioglossoides L2 from Fruit Body and Optimization of Fermentation Conditions for Its Mycelial Growth

DOI：10.1016/S1004-9541(08)60206-2

摘要

Abstract

关键词

Keywords

references