Efficient synthesis of tyrosol from L-tyrosine <i>via</i> heterologous Ehrlich pathway in <i>Escherichia coli</i>

Xiaobo Ruan; Sheng Zhang; Wei Song; Jia Liu; Xiulai Chen; Liming Liu; Jing Wu

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Efficient synthesis of tyrosol from L-tyrosine via heterologous Ehrlich pathway in Escherichia coli

Full Length Article | Updated：2026-01-06

- Efficient synthesis of tyrosol from L-tyrosine via heterologous Ehrlich pathway in Escherichia coli
- Efficient synthesis of tyrosol from L-tyrosine via heterologous Ehrlich pathway in Escherichia coli
- 中国化学工程学报（英文版） 2022年47卷第7期页码：18-30
- Affiliations：
  
  1. School of Pharmaceutical Science, Jiangnan University,Wuxi,China,214122
  2. State Key Laboratory of Food Science and Technology, Jiangnan University,Wuxi,China,214122
  3. Tianrui Chemical Co., Ltd, Department of Chemistry,Quzhou,China,324400
- Author bio：
- Funds：
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  中图分类号：
- 纸质出版：2022
- Accepted：
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Xiaobo Ruan, Sheng Zhang, Wei Song, 等. Efficient synthesis of tyrosol from L-tyrosine via heterologous Ehrlich pathway in Escherichia coli[J]. 中国化学工程学报（英文版）, 2022,47(7):18-30.

heterologous Ehrlich pathway in
Xiaobo Ruan, Sheng Zhang, Wei Song, 等. Efficient synthesis of tyrosol from L-tyrosine via heterologous Ehrlich pathway in Escherichia coli[J]. 中国化学工程学报（英文版）, 2022,47(7):18-30. DOI：

heterologous Ehrlich pathway in DOI：

摘要

Abstract

For the efficient conversion of L-tyrosine (L-Tyr) to tyrosol

which is an aromatic compound widely used in the pharmaceutical and chemical industries

a novel four-enzyme cascade pathway based on the Ehrlich pathway of

Saccharomyces cerevisiae

was designed and reconstructed in

Escherichia coli

. Then

the expression levels of the relevant enzymes were coordinated using a modular approach and gene duplication after the identification of the pyruvate decarboxylase from

Candida tropicalis

(

PDC) as the rate-limiting enzymatic step.

In situ

product removal (ISPR) strategy with XAD4 resins was explored to avoid product inhibition and further improve tyrosol yield. As a result

the titer and conversion rate of tyrosol obtained were 35.7 g·L

-1

and 93.6%

respectively

in a 3-L bioreactor. Results presented here provide a potential enzymatic process for industrial production of tyrosol from cheap amino acids.

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Efficient synthesis of tyrosol from L-tyrosine via heterologous Ehrlich pathway in Escherichia coli

Efficient synthesis of tyrosol from L-tyrosine via heterologous Ehrlich pathway in Escherichia coli

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摘要

Abstract

关键词

Keywords

references