Asymmetric bioreduction of γ- and δ-keto acids by native carbonyl reductases from <i>Saccharomyces cerevisiae</i>

Chunlei Ren; Tao Wang; Xiaoyan Zhang; Jiang Pan; Jianhe Xu; Yunpeng Bai

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Asymmetric bioreduction of γ- and δ-keto acids by native carbonyl reductases from Saccharomyces cerevisiae

Special Topic: Biocatalysis | Updated：2026-01-06

- Asymmetric bioreduction of γ- and δ-keto acids by native carbonyl reductases from Saccharomyces cerevisiae
- Asymmetric bioreduction of γ- and δ-keto acids by native carbonyl reductases from Saccharomyces cerevisiae
- 中国化学工程学报（英文版） 2021年29卷第1期页码：305-310
- Affiliations：
  
  1. State Key Laboratory of Bioreactor Engineering
  2. Shanghai Collaborative Innovation Centre for Biomanufacturing, East China University of Science and Technology,Shanghai,China,200237
- Author bio：
- Funds：
- DOI：
  中图分类号：
- 纸质出版：2021
- Accepted：
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Chunlei Ren, Tao Wang, Xiaoyan Zhang, 等. Asymmetric bioreduction of γ- and δ-keto acids by native carbonyl reductases from Saccharomyces cerevisiae[J]. 中国化学工程学报（英文版）, 2021,29(1):305-310.

[J]. 中国化学工程学报, 2021, 29(1): 305-310.
Chunlei Ren, Tao Wang, Xiaoyan Zhang, 等. Asymmetric bioreduction of γ- and δ-keto acids by native carbonyl reductases from Saccharomyces cerevisiae[J]. 中国化学工程学报（英文版）, 2021,29(1):305-310. DOI：

[J]. 中国化学工程学报, 2021, 29(1): 305-310. DOI：

摘要

Abstract

Optically pure (

- and (

-lactones can be prepared by intramolecular cyclization of chiral hydroxy acids/esters reduced asymmetrically from γ- and δ-keto acids/esters using

Saccharomyces cerevisiae

(

S. cerevisiae

) as a whole-cell biocatalyst. However

some of the enzymes catalyzing these reactions in

S. cerevisiae

are still unknown up to date. In this report

two carbonyl reductases

OdCR1 and OdCR2

were successfully discovered

and cloned from

S. cerevisiae

using a genome-mining approach

and overexpressed in

Escherichia coli

(

E. coli

). Compared with OdCR1

OdCR2 can reduce 4-oxodecanoic acid and 5-oxodecanoic acid asymmetrically with higher stereoselectivity

generating (

-decalactone (99% ee) and (

-decalactone (98%

) in 85% and 92% yields

respectively. This is the first report of native enzymes from

S. cerevisiae

for the enzymatic synthesis of chiral γ- and δ-lactones which is of wide uses in food and cosmetic industries.

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Asymmetric bioreduction of γ- and δ-keto acids by native carbonyl reductases from Saccharomyces cerevisiae

Asymmetric bioreduction of γ- and δ-keto acids by native carbonyl reductases from Saccharomyces cerevisiae

DOI：

摘要

Abstract

关键词

Keywords

references