Fabrication and characterization of epoxylated zwitterionic copolymergrafted silica nanoparticle as a new support for lipase immobilization

Ning Chen; Chunyu Zhang; Xiaoyan Dong; Yan Sun

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Fabrication and characterization of epoxylated zwitterionic copolymergrafted silica nanoparticle as a new support for lipase immobilization

Biotechnology and Bioengineering | Updated：2026-01-06

- Fabrication and characterization of epoxylated zwitterionic copolymergrafted silica nanoparticle as a new support for lipase immobilization
- Fabrication and characterization of epoxylated zwitterionic copolymergrafted silica nanoparticle as a new support for lipase immobilization
- 中国化学工程学报（英文版） 2020年28卷第4期页码：1129-1135
- Affiliations：
  
  Department of Biochemical Engineering and Key Laboratory of Systems Bioengineering (Ministry of Education), School of Chemical Engineering and Technology, Tianjin University,Tianjin,China,300354
- Author bio：
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- 纸质出版：2020
- Accepted：
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Ning Chen, Chunyu Zhang, Xiaoyan Dong, 等. Fabrication and characterization of epoxylated zwitterionic copolymergrafted silica nanoparticle as a new support for lipase immobilization[J]. 中国化学工程学报（英文版）, 2020,28(4):1129-1135.

Ning Chen, Chunyu Zhang, Xiaoyan Dong, Yan Sun. Fabrication and characterization of epoxylated zwitterionic copolymergrafted silica nanoparticle as a new support for lipase immobilization[J]. Chinese Journal of Chemical Engineering, 2020, 28(4): 1129-1135.
Ning Chen, Chunyu Zhang, Xiaoyan Dong, 等. Fabrication and characterization of epoxylated zwitterionic copolymergrafted silica nanoparticle as a new support for lipase immobilization[J]. 中国化学工程学报（英文版）, 2020,28(4):1129-1135. DOI：

Ning Chen, Chunyu Zhang, Xiaoyan Dong, Yan Sun. Fabrication and characterization of epoxylated zwitterionic copolymergrafted silica nanoparticle as a new support for lipase immobilization[J]. Chinese Journal of Chemical Engineering, 2020, 28(4): 1129-1135. DOI：

摘要

Abstract

Our previous work proved that the thermal stability of

Candida rugosa

lipase (CRL) immobilized on zwitterionic polymer (poly(carboxybetaine methacrylate)) grafted silica nanoparticle (SNP) was much higher than that on poly(glycidyl methecrylate) (pGMA) grafted SNP

while the latter showed significantly increased activity. Inspired by the research

we have herein proposed to synthesize copolymers of zwitterionic sulfobetaine methacrylate (SBMA) and GMA for CRL immobilization. The copolymers were grafted onto SNP surface at three GMA/ SBMA (G/S) molar ratios (G100/S0

G50/S50

G10/S90)

followed by the covalent coupling of CRL to the surface copolymers. The immobilized CRLs on the corresponding supports were denoted as p(G100-S0)-CRL

p(G50-S50)-CRL and p(G10-S90)-CRL. The enzyme loading increased with the increase of GMA content in the copolymer

while the activity varied with the grafted copolymer composition. Kinetic study proved the improvement of enzyme-substrate affinity after immobilization. In comparison to p(G100-S0)-CRL

p(G50-S50)-CRL and p (G10-S90)-CRL presented remarkably enhanced thermal stability and pH tolerance

and p(G10-S90)-CRL

showed the highest stability. These results suggest that the copolymer design is promising for development as a versatile platform for enzyme immobilization.

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