Immobilization of organophosphorus hydrolase enzyme by covalent attachment on modified cellulose microfibers using different chemical activation strategies: Characterization and stability studies

Meisam Sharifi; Seyed-Mortaza Robatjazi; Minoo Sadri; Jafar Mohammadian Mosaabadi

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Immobilization of organophosphorus hydrolase enzyme by covalent attachment on modified cellulose microfibers using different chemical activation strategies: Characterization and stability studies

Biotechnology and Bioengineering | Updated：2026-01-06

- Immobilization of organophosphorus hydrolase enzyme by covalent attachment on modified cellulose microfibers using different chemical activation strategies: Characterization and stability studies
- Immobilization of organophosphorus hydrolase enzyme by covalent attachment on modified cellulose microfibers using different chemical activation strategies: Characterization and stability studies
- 中国化学工程学报（英文版） 2019年27卷第1期页码：191-199
- Affiliations：
  
  Department of Bioscience and Biotechnology, Malek Ashtar University of Technology, Tehran,Iran
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- 纸质出版：2019
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Meisam Sharifi, Seyed-Mortaza Robatjazi, Minoo Sadri, 等. Immobilization of organophosphorus hydrolase enzyme by covalent attachment on modified cellulose microfibers using different chemical activation strategies: Characterization and stability studies[J]. 中国化学工程学报（英文版）, 2019,27(1):191-199.

Meisam Sharifi, Seyed-Mortaza Robatjazi, Minoo Sadri, Jafar Mohammadian Mosaabadi. Immobilization of organophosphorus hydrolase enzyme by covalent attachment on modified cellulose microfibers using different chemical activation strategies: Characterization and stability studies[J]. Chin.J.Chem.Eng., 2019, 27(1): 191-199.
Meisam Sharifi, Seyed-Mortaza Robatjazi, Minoo Sadri, 等. Immobilization of organophosphorus hydrolase enzyme by covalent attachment on modified cellulose microfibers using different chemical activation strategies: Characterization and stability studies[J]. 中国化学工程学报（英文版）, 2019,27(1):191-199. DOI：

Meisam Sharifi, Seyed-Mortaza Robatjazi, Minoo Sadri, Jafar Mohammadian Mosaabadi. Immobilization of organophosphorus hydrolase enzyme by covalent attachment on modified cellulose microfibers using different chemical activation strategies: Characterization and stability studies[J]. Chin.J.Chem.Eng., 2019, 27(1): 191-199. DOI：

摘要

Abstract

The plant cellulose powder was activated by two different methods using 1

4-butanediol diglycidyl ether (BTDE) and 1

1'-Carbonyldiimidazole (CDI) as the chemical coupling agents. Organophosphorus hydrolase (OPH) from Flavobacterium ATCC 27551 was immobilized on any of activated support through covalent bonding. The optimal conditions of affecting parameters on enzyme immobilization in both methods were found

and it was demonstrated that the highest activity yields of immobilized OPH onto epoxy and CDI treated cellulose were 68.32% and 73.51%

respectively. The surface treatment of cellulose via covalent coupling with BTDE and CDI agents was proved by FTIR analysis. The kinetic constants of the free and immobilized enzymes were determined

and it was showed that both immobilization techniques moderately increased the K

value of the free OPH. The improvements in storage and thermal stability were investigated and depicted that the half-life of immobilized OPH over the surface of epoxy modified cellulose had a better growth compared to the free and immobilized enzymes onto CDI treated supp

ort. Also

the pH stability of the immobilized preparations was enhanced relative to the free counterpart and revealed that all enzyme samples would have the same optimum pH value for stability at 9.0. Additionally

the immobilized OPH onto epoxy and CDI activated cellulose retained about 59% and 68% of their initial activity after ten turns of batch operation

respectively. The results demonstrated the high performance of OPH enzyme in immobilized state onto an inexpensive support with the potential of industrial applications.

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