Experimental and in silico studies on three hydrophobic charge-induction adsorbents for porcine immunoglobulin purification

Qilei Zhang; Tiantian Zhuang; Hongfei Tong; Hongyin Wang; Dongqiang Lin; Shanjing Yao

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Experimental and in silico studies on three hydrophobic charge-induction adsorbents for porcine immunoglobulin purification

第25届中国过程控制会议专栏 | Updated：2026-01-06

- Experimental and in silico studies on three hydrophobic charge-induction adsorbents for porcine immunoglobulin purification
- Experimental and in silico studies on three hydrophobic charge-induction adsorbents for porcine immunoglobulin purification
- 中国化学工程学报（英文版） 2016年24卷第1期页码：151-157
- Affiliations：
  
  Key Laboratory of Biomass Chemical Engineering of Ministry of Education, Department of Chemical and Biological Engineering, Zhejiang University,Hangzhou,China,310027
- Author bio：
- Funds：
- DOI：
  中图分类号：
- 纸质出版：2016
- Accepted：
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Qilei Zhang, Tiantian Zhuang, Hongfei Tong, 等. Experimental and in silico studies on three hydrophobic charge-induction adsorbents for porcine immunoglobulin purification[J]. 中国化学工程学报（英文版）, 2016,24(1):151-157.

Qilei Zhang, Tiantian Zhuang, Hongfei Tong, Hongyin Wang, Dongqiang Lin, Shanjing Yao. Experimental and in silico studies on three hydrophobic charge-induction adsorbents for porcine immunoglobulin purification[J]. Chin.J.Chem.Eng., 2016, 24(1): 151-157.
Qilei Zhang, Tiantian Zhuang, Hongfei Tong, 等. Experimental and in silico studies on three hydrophobic charge-induction adsorbents for porcine immunoglobulin purification[J]. 中国化学工程学报（英文版）, 2016,24(1):151-157. DOI：

Qilei Zhang, Tiantian Zhuang, Hongfei Tong, Hongyin Wang, Dongqiang Lin, Shanjing Yao. Experimental and in silico studies on three hydrophobic charge-induction adsorbents for porcine immunoglobulin purification[J]. Chin.J.Chem.Eng., 2016, 24(1): 151-157. DOI：

摘要

Abstract

Three hydrophobic charge-induction adsorbents with functional ligands of 4-mercapto-ethyl-pyridine

2-mercapto-methyl-imidazole or 2-mercapto-benzimidazole were evaluated in the purification of porcine immunoglobulin from porcine blood. Adsorption isothermswere studied under different pH conditions. The adsorbent with 2-mercapto-methyl-imidazole as the ligand showed reasonable adsorption capacity (43.60 mg·g

-1

gel) with great selectivity and it also showed the best elution performance in chromatographic studies. A multi-pH step elution process was proposed for the 2-mercapto-methyl-imidazole adsorbent

and the results showed that high immunoglobulin purity (94.3%) and a yield of 9.8 mg·(ml plasma)

-1

could be achieved under the optimal condition of loading (pH 5.0)-pre-elution (pH 7.0)-elution (pH 3.8). Moreover

molecular simulation was employed to help in analyzing the binding mechanism between the ligands and immunoglobulin

and the results showed that both 2-mercapto-benzimidazole and 2-mercapto-methyl-imidazole ligands were docked on the same pocket (around TYR319 and LEU309) of the Fc fragment of immunoglobulin

with 2-mercaptobenzimidazole showing stronger binding interactions.

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