Isolation and Characterization of Heterotrophic Nitrifying Strain W1<sup>*</sup>

吕永康; 王荀; 刘博凯; 刘玉香; 杨晓华

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Isolation and Characterization of Heterotrophic Nitrifying Strain W1^*

Updated：2026-01-06

- Isolation and Characterization of Heterotrophic Nitrifying Strain W1^*
- Isolation and Characterization of Heterotrophic Nitrifying Strain W1^*
- 中国化学工程学报（英文版） 2012年20卷第5期页码：995-1002
- Affiliations：
  
  1. Key Laboratory of Coal Science and Technology, Taiyuan University of Technology,Taiyuan,China,030024
  2. Engineering & the Built Environment (EBE), University of Cape Town, Cape Town7701, South Africa
  3. College of Environmental Science and Engineering, Taiyuan University of Technology,Taiyuan,China,030024
- Author bio：
- Funds：
  
  Supported by the National Natural Science Foundation of China (51078252);the International Cooperation Projects of Shanxi Province (2010081018);the Natural Science Foundation of Shanxi Province (2010011016-1)
- DOI：
  中图分类号：
- 纸质出版：2012
- Accepted：
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吕永康, 王荀, 刘博凯, 等. Isolation and Characterization of Heterotrophic Nitrifying Strain W1^*[J]. 中国化学工程学报（英文版）, 2012,20(5):995-1002.

[J]. Chinese Journal of Chemical Engineering, 2012, 20(5): 995-1002.
吕永康, 王荀, 刘博凯, 等. Isolation and Characterization of Heterotrophic Nitrifying Strain W1^*[J]. 中国化学工程学报（英文版）, 2012,20(5):995-1002. DOI：

[J]. Chinese Journal of Chemical Engineering, 2012, 20(5): 995-1002. DOI：

摘要

Abstract

In a high concentration substrate medium

a heterotrophic bacterium with high removal efficiency of ammonium

named W1

was isolated from activated sludge of coking wastewater treatment facility. The bacterium was Gram-negative

rod-shaped

and identified preliminarily as

Alcaligenes

sp. according to its morphological and physiological properties and its 16S rRNA gene sequence analysis. In the high concentration ammonium medium (400 mg·L

-1

-N)

the effects of C source

N source

C/N ratio and initial pH of medium on ammonium removal were investigated in order to determine the optimal condition for strain W1. The maximum ammonium removal was around 95% in 4 days in an improved medium. The production of N

gas was examined in a closed system that was full of pure oxygen at the beginning. N

gas was detected in the system after 4 days of cultivation

which further testified that strain W1 has heterotrophic nitrification and aerobic denitrification abilities simultaneously.

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