Optimizing the Chemical Compositions of Protective Agents for Freeze-drying <i>Bifidobacterium longum</i> BIOMA 5920<sup>*</sup>

杨婵媛; 朱晓丽; 范代娣; 米钰; 骆艳娥; 惠俊峰; 苏然

Your Location：

Home >

Browse articles >

Optimizing the Chemical Compositions of Protective Agents for Freeze-drying Bifidobacterium longum BIOMA 5920^*

Updated：2026-01-06

- Optimizing the Chemical Compositions of Protective Agents for Freeze-drying Bifidobacterium longum BIOMA 5920^*
- Optimizing the Chemical Compositions of Protective Agents for Freeze-drying Bifidobacterium longum BIOMA 5920^*
- 中国化学工程学报（英文版） 2012年20卷第5期页码：930-936
- Affiliations：
  
  1. Shaanxi Key Laboratory of Degradable Biomedical Materials, School of Chemical Engineering, Northwest University,Xi'an,China,710069
  2. Shaanxi R&D Center of Biomaterials and Fermentation Engineering, School of Chemical Engineering, Northwest University,Xi'an,China,710069
  3. Shaanxi R&D Center of Biomaterials and Fermentation Engineering, School of Urban and Environmental Science, Northwest University,Xi'an,China,710069
- Author bio：
- Funds：
  
  Supported by the National High Technology Research and Development Program of China (2007AA03Z456);the National Natural Science Foundation of China (20776119, 21076169 and 31000019);the Xi’an Research and Development Program(NC08005, YF07078);the Scientific Research Program of Shaanxi Provincial Department of Education,China(08JK452,08JK453,JG08181,2010JC21,2010JS107,2010JS108, 2010JK876 and 2010JS109);Shaanxi Provincial Scientific Technology Research and Development Program (2007K06-03, 2010JQ2012, SJ08B03);the Specialized Research Fund for the Doctoral Program of Higher Education of China (20096101120023, 20096101110014);NWU Graduate Innovation and Creativity Funds (08YSY17);Shaanxi Key Subject Program, China
- DOI：
  中图分类号：
- 纸质出版：2012
- Accepted：
Scan QR Code
杨婵媛, 朱晓丽, 范代娣, 等. Optimizing the Chemical Compositions of Protective Agents for Freeze-drying Bifidobacterium longum BIOMA 5920^*[J]. 中国化学工程学报（英文版）, 2012,20(5):930-936.

BIOMA 5920
杨婵媛, 朱晓丽, 范代娣, 等. Optimizing the Chemical Compositions of Protective Agents for Freeze-drying Bifidobacterium longum BIOMA 5920^*[J]. 中国化学工程学报（英文版）, 2012,20(5):930-936. DOI：

BIOMA 5920 DOI：

摘要

Abstract

Freeze drying has a deleterious effect on the viability of microorganisms. In front of this difficulty

the present study adopts response surface methodology to optimize the chemical compositions of protective agents to seek for maximum viability of

Bifidobacterium longum

BIOMA 5920 during freeze-drying. Through the comparative analysis of single protectant

the complex protective agents show better effect on the

Bifidobacterium

viability. Human-like collagen (HLC)

trehalose and glycerol are confirmed as significant factors by Box-Behnken Design. The optimized formula for these three variables is tested as follows: HLC 1.23%

trehalose 11.50% and glycerol 4.65%. Under this formula

the viability is 88.23%

39.67% higher in comparison to the control. The viable count is 1.07×10

cfu·g

-1

greatly exceeding the minimum viable count requirement (10

cfu·g

-1

关键词

Keywords

references

浏览量

398

Downloads

CSCD

文章被引用时，请邮件提醒。

Submit

工具集

关联资源

Microfluidic extraction of phenolic compounds from wastewater in the straight/helical/in-plane spiral microchannels

Optimization of methanol distillation process using response surface methodology

Microwave-assisted dehydration of strontium hydroxide octahydrate: Experimental study and kinetic modeling analysis

Box-Behnken experimental design for optimizing process parameters in carbonate-promoted direct thiophene carboxylation reaction with carbon dioxide

Evaluation of phenanthrene removal from soil washing effluent by activated carbon adsorption using response surface methodology

Optimizing the Chemical Compositions of Protective Agents for Freeze-drying Bifidobacterium longum BIOMA 5920^*

Optimizing the Chemical Compositions of Protective Agents for Freeze-drying Bifidobacterium longum BIOMA 5920^*

DOI：

摘要

Abstract

关键词

Keywords

references

Optimizing the Chemical Compositions of Protective Agents for Freeze-drying Bifidobacterium longum BIOMA 5920*

Optimizing the Chemical Compositions of Protective Agents for Freeze-drying Bifidobacterium longum BIOMA 5920*

DOI：

摘要

Abstract

关键词

Keywords

references

Optimizing the Chemical Compositions of Protective Agents for Freeze-drying Bifidobacterium longum BIOMA 5920^*

Optimizing the Chemical Compositions of Protective Agents for Freeze-drying Bifidobacterium longum BIOMA 5920^*