Synthesis of Wax Esters by Lipase-catalyzed Esterification with Immobilized Lipase from <i>Candida</i> sp. 99-125

邓利; 王晓静; 聂开立; 王芳; 刘军峰; 王璞; 谭天伟

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Synthesis of Wax Esters by Lipase-catalyzed Esterification with Immobilized Lipase from Candida sp. 99-125

Updated：2026-01-06

- Synthesis of Wax Esters by Lipase-catalyzed Esterification with Immobilized Lipase from Candida sp. 99-125
- Synthesis of Wax Esters by Lipase-catalyzed Esterification with Immobilized Lipase from Candida sp. 99-125
- 中国化学工程学报（英文版） 2011年19卷第6期页码：978-982
- Affiliations：
  
  1. College of Life Science and Technology, Beijing Key Laboratory of Bioprocess, Biorefinery Research and Engineering Center, Ministry of Education, Beijing University of Chemical Technology,Beijing,China,100029
  2. College of Chemical Engineering, Beijing University of Chemical Technology,Beijing,China,100029
- Author bio：
- Funds：
  
  Supported by the National High Technology Research and Development Program of China (2009AA02Z207);the National Natural Science Foundation of China (20876012);the Hi-Tech Research and Development Program of China (2009AA03Z232,2010AA101601)
- DOI：
  中图分类号：
- 纸质出版：2011
- Accepted：
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邓利, 王晓静, 聂开立, 等. Synthesis of Wax Esters by Lipase-catalyzed Esterification with Immobilized Lipase from Candida sp. 99-125[J]. 中国化学工程学报（英文版）, 2011,19(6):978-982.

sp. 99-125[J]. , 2011, 19(6): 978-982.
邓利, 王晓静, 聂开立, 等. Synthesis of Wax Esters by Lipase-catalyzed Esterification with Immobilized Lipase from Candida sp. 99-125[J]. 中国化学工程学报（英文版）, 2011,19(6):978-982. DOI：

sp. 99-125[J]. , 2011, 19(6): 978-982. DOI：

摘要

Abstract

Wax esters were synthesized in a solvent free system catalyzed by immobilized lipase from

Candida

sp. 99-125

with oleic acid and cetyl alcohol. The effects of substrate molar ratio

lipase dosage and water removal were investigated in a 50 ml flask incubated in a thermostatic cultivation cabinet. The optimized conditions were: temperature 40℃

shaking at 170 r·min

-1

acid/alcohol molar ratio 1:0.9

lipase dosage in 10% (by mass) of oleic acid

and open reaction for water removal. As a result

the conversion rate reached 98% for reaction of 8 h. The volume of reactor was scaled up to 1 L three-neck flask. The optimized parameters were: 200 r·min

-1

agitation speed

2.5% (by mass) lipase dosage

others were the same as the parameters described above. The conversion rate reached 95% for reaction of 24 h. The lipase retained 46% conversion rate after reuse for 6

7 batches. The products were purified by removing remained cetyl alcohol and fatty acids with ethanol and saturated sodium carbonate solution

respectively. The purity of the wax ester

cetyl oleate

was 96%. The physical and chemical properties of cetyl oleate were tested and compared with those of jojoba oil. The results show that the product cetyl oleate has great potential to use as the substitute of natural jojoba oil.

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Synthesis of Wax Esters by Lipase-catalyzed Esterification with Immobilized Lipase from Candida sp. 99-125

Synthesis of Wax Esters by Lipase-catalyzed Esterification with Immobilized Lipase from Candida sp. 99-125

DOI：

摘要

Abstract

关键词

Keywords

references