Elementary flux mode (EFM) analysis was used in the metabolic analysis of central carbon metabolism in
Saccharomyces cerevisiae
based on constructed cellular network. Calculated from the metabolic model
the ethanol-producing pathway No. 37 furthest converts the substrate into ethanol among the 78 elementary flux modes. The in silico metabolic phenotypes predicted based on this analysis fit well with the fermentation performance of the engineered strains
KAM3 and KAM11
which confirmed that EFM analysis is valid to direct the construction of
Saccharomyces cerevisiae
engineered strains
to increase the ethanol yield.
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Design of a rhodopsin-based light-driven proton pump to improve acid tolerance of yeast
Acetic acid- and furfural-based adaptive evolution of Saccharomyces cerevisiae strains for improving stress tolerance and lignocellulosic ethanol production
相关作者
Omama Rehman
Jingfang Chen
Quan Zhang
Muzi Hu
Cuihuan Sun
Youduo Wu
Huipeng Gao
Muhammad Jawad
相关机构
School of Chemical Engineering, Dalian University of Technology
School of Chemical Engineering, Zhengzhou University
C1 Refinery Engineering Research Center, Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences
Microbial Research Institute of Liaoning Province
SINOPEC Dalian Research Institute of Petroleum and Petrochemicals Co., Ltd.