Mining and engineering of terpene synthases and their applications in biomanufacturing

Yue Xu; Chenwen Liu; Lei Qin; Bo Lv; Genlin Zhang; Chun Li

doi:10.1016/j.cjche.2025.08.001

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Mining and engineering of terpene synthases and their applications in biomanufacturing

Special Issue on Celebrating the 100th Anniversary of the School of Chemical Engineering and Technology of Tianjin University | Updated：2026-01-08

- Mining and engineering of terpene synthases and their applications in biomanufacturing
- Chinese Journal of Chemical Engineering Vol. 86, Issue 10, Pages: 138-149(2025)
- Affiliations：
  
  1. Key Laboratory for Green Processing of Chemical Engineering of Xinjiang Bingtuan, School of Chemistry and Chemical Engineering, Shihezi University,Shihezi,China,832003
  2. Department of Chemical Engineering, Tsinghua University,Beijing,China,100084
  3. Key Lab for Industrial Biocatalysis, Ministry of Education, Tsinghua University,Beijing,China,100084
  4. Center for Synthetic and Systems Biology, Tsinghua University,Beijing,China,100084
  5. Beijing Key Laboratory of Recombinant Protein Synthetic Biomanufacturing,Beijing,China,100084
  6. State Key Laboratory of Green Biomanufacturing,Beijing,China,100084
  7. Key Laboratory of Medical Molecule Science and Pharmaceutics Engineering, Ministry of Industry and Information Technology, Institute of Biochemical Engineering, School of Chemistry and Chemical Engineering, Beijing Institute of Technology,Beijing,China,100081
- Author bio：
- Funds：
- DOI：10.1016/j.cjche.2025.08.001
  CLC：
- Published：2025
- Accepted：
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Yue Xu, Chenwen Liu, Lei Qin, Bo Lv, Genlin Zhang, Chun Li. Mining and engineering of terpene synthases and their applications in biomanufacturing[J]. Chinese Journal of Chemical Engineering, 2025, 86(10): 138-149.
DOI：

Yue Xu, Chenwen Liu, Lei Qin, Bo Lv, Genlin Zhang, Chun Li. Mining and engineering of terpene synthases and their applications in biomanufacturing[J]. Chinese Journal of Chemical Engineering, 2025, 86(10): 138-149. DOI： 10.1016/j.cjche.2025.08.001.

摘要

Abstract

Terpenoids

one of the most diverse and structurally varied natural products in nature

are widely distributed in plants

microbes

and other organisms. Their structural diversity confers significant importance in medicine

food

flavorings

and energy. However

traditional methods of plant extraction and chemical synthesis have limitations in industrial applications. Consequently

microbial cell factories have emerged as an important platform for terpenoid production. Terpene synthases (TPSs) are crucial in determining the structural and functional diversity of terpenoids. This review discussed the origin and classification of TPSs

outlines commonly used TPS mining methods

and summarizes advances in TPS engineering. In addition

it also explores the influence of machine learning on enzyme mining

the existing challenges and the future opportunities alongside cutting-edge technologies.

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Related Institution

Center for Synthetic & Systems Biology, Tsinghua University

Key Laboratory for Industrial Biocatalysis, Ministry of Education, Department of Chemical Engineering, Tsinghua University

School of Chemical Engineering and Technology, Xi'an Key Laboratory of C1 Compound Bioconversion Technology, Xi'an Jiaotong University

Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences

Key Lab for Industrial Biocatalysis, Ministry of Education, Department of Chemical Engineering, Tsinghua University

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