Facile purification and immobilization of organophosphorus hydrolase on protein-inorganic hybrid phosphate nanosheets

Zhenfu Wang; Jie Gao; Qinghong Shi; Xiaoyan Dong; Yan Sun

doi:10.1016/j.cjche.2022.07.017

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Facile purification and immobilization of organophosphorus hydrolase on protein-inorganic hybrid phosphate nanosheets

Full Length Article | Updated：2026-01-06

- Facile purification and immobilization of organophosphorus hydrolase on protein-inorganic hybrid phosphate nanosheets
- Chinese Journal of Chemical Engineering Vol. 56, Issue 4, Pages: 119-125(2023)
- Affiliations：
  
  Department of Biochemical Engineering, School of Chemical Engineering and Technology and Key Laboratory of Systems Bioengineering and Frontiers Science Center for Synthetic Biology (Ministry of Education), Tianjin University,Tianjin,China,300350
- Author bio：
- Funds：
- DOI：10.1016/j.cjche.2022.07.017
  CLC：
- Published：2023
- Accepted：
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Zhenfu Wang, Jie Gao, Qinghong Shi, Xiaoyan Dong, Yan Sun. Facile purification and immobilization of organophosphorus hydrolase on protein-inorganic hybrid phosphate nanosheets[J]. Chinese Journal of Chemical Engineering, 2023, 56(4): 119-125.
DOI：

Zhenfu Wang, Jie Gao, Qinghong Shi, Xiaoyan Dong, Yan Sun. Facile purification and immobilization of organophosphorus hydrolase on protein-inorganic hybrid phosphate nanosheets[J]. Chinese Journal of Chemical Engineering, 2023, 56(4): 119-125. DOI： 10.1016/j.cjche.2022.07.017.

摘要

Abstract

Oriented immobilization of enzymes helps to maintain their native structure and proper orientation for high-performance engineering to meet extensive biocatalysis demands. However

the supporting materials used for orientated immobilization are usually costly or complicated in preparation

affecting their practical applications. In this work

a facile purification and immobilization method was proposed for enzyme immobilization based on organic–inorganic hybrid calcium phosphate nanocrystal (CaPs) induced by Cu

modified bovine serum albumin (BSA-Cu). Then

the as-prepared hybrid calcium phosphate nanosheet

BSA-Cu@CaPs

was utilized for one-pot purification and immobilization of His-tagged organophosphorus hydrolase (OPH) by metal-affinity binding to the incorporated BSA. BSA-Cu@CaPs-OPH exhibited enhanced pH stability and thermal stability compared to the free enzyme. Moreover

BSA-Cu@CaPs-OPH could retain more than 75% and 56% of initial activity after reuse 5 and 10 times

respectively. The results demonstrated that this facile strategy was promising for the effective biodegradation of organophosphorus pesticides with the immobilized enzyme.

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