Highly efficient synthesis of (R)-1,3-butanediol via anti-Prelog reduction of 4-hydroxy-2-butanone with absolute stereoselectivity by a newly isolated Pichia kudriavzevii

Han Zu; Hui Zhang; Anwen Fan; Jie Gu; Yao Nie; Pengjie Luo; Yan Xu

doi:10.1016/j.cjche.2019.12.015

Your Location：

Home >

Browse articles >

Highly efficient synthesis of (R)-1,3-butanediol via anti-Prelog reduction of 4-hydroxy-2-butanone with absolute stereoselectivity by a newly isolated Pichia kudriavzevii

Biotechnology and Bioengineering | Updated：2026-01-06

- Highly efficient synthesis of (R)-1,3-butanediol via anti-Prelog reduction of 4-hydroxy-2-butanone with absolute stereoselectivity by a newly isolated Pichia kudriavzevii
- Chinese Journal of Chemical Engineering Vol. 28, Issue 8, Pages: 2160-2166(2020)
- Affiliations：
  
  1. School of Biotechnology and Key Laboratory of Industrial Biotechnology of Ministry of Education, Jiangnan University,Wuxi,China,214122
  2. China National Center for Food Safety Risk Assessment, NHC Key Laboratory of Food Safety Risk Assessment,Beijing,China,100022
  3. State Key Laboratory of Food Science and Technology, Jiangnan University,Wuxi,China,214122
- Author bio：
- Funds：
- DOI：10.1016/j.cjche.2019.12.015
  CLC：
- Published：2020
- Accepted：
Scan QR Code
)-1,3-butanediol
DOI：

)-1,3-butanediol DOI： 10.1016/j.cjche.2019.12.015.

摘要

Abstract

(

)-1

3-butanediol is an important pharmaceutical intermediate

and the synthesis of (

)-1

3-butanediol using green biological methods has recently been of interest for industrial application. Here

a novel strain QC-1 that efficiently transforms 4-hydroxy-2-butanone to (

)-1

3-butanediol was isolated from soil samples. Based on morphological

physiological

and biochemical tests and 5.8S-internal transcribed spacer sequencing

the strain was identified as

Pichia kudriavzevii

QC-1. The reaction conditions were optimized to 35 ℃

pH 8.0

rotation speed 200 rpm

and 6:5 mass ratio of glucose to 4-hydroxy-2-butanone. Evaluation of the effects of 4-hydroxy- 2-butanone concentrations on yield and cell survival rate showed that 85.60 g·L

-1

product accumulated

with an enantiomeric excess of more than 99%

when 30 g·L

-1

4-hydroxy-2-butanone was added at 0

and 30 h in a 3-L bioreactor. Thus

strain QC-1 showed excellent catalytic activity and stereoselectivity for the synthesis of (

)-1

3-butanediol from 4-hydroxy-2-butanone.

关键词

Keywords

references

Views

258

Downloads

CSCD

Alert me when the article has been cited

Submit

Tools

Publicity Resources

Asymmetric bioreduction of γ- and δ-keto acids by native carbonyl reductases from Saccharomyces cerevisiae

Salting-out Extraction of 2,3-Butanediol from Jerusalem artichoke-based Fermentation Broth

Asymmetric Bioreduction of 3,5-Bis(trifluoromethyl) Acetophenone to Its Corresponding Alcohol by Candida tropicalis

Production of Chiral Aromatic Alcohol by Asymmetric Reduction with Vegetable Catalyst

Reaction Characteristics of Asymmetric Synthesis of (2S,5S)-2,5-Hexanediol Catalyzed with Baker’s Yeast Number 6

Related Author

Yunpeng Bai

Jianhe Xu

Jiang Pan

Xiaoyan Zhang

Tao Wang

Chunlei Ren

修志龙

张媛丽

Related Institution

State Key Laboratory of Bioreactor Engineering

Shanghai Collaborative Innovation Centre for Biomanufacturing, East China University of Science and Technology

School of Life Science and Biotechnology, Dalian University of Technology

College of Pharmaceutical Science, Zhejiang University of Technology

Zhejiang Pharmaceutical College

⁰