Salt-tolerant mechanism of marine <i>Aspergillus niger</i> cellulase cocktail and improvement of its activity

Linian Cai; Shengnan Xu; Tao Lu; Dongqiang Lin; Shanjing Yao

doi:10.1016/j.cjche.2019.11.012

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Salt-tolerant mechanism of marine Aspergillus niger cellulase cocktail and improvement of its activity

Biotechnology and Bioengineering | Updated：2026-01-06

- Salt-tolerant mechanism of marine Aspergillus niger cellulase cocktail and improvement of its activity
- Chinese Journal of Chemical Engineering Vol. 28, Issue 4, Pages: 1120-1128(2020)
- Affiliations：
  
  1. Key Laboratory of Biomass Chemical Engineering of Ministry of Education, College of Chemical and Biological Engineering, Zhejiang University,Hangzhou,China,310027
  2. College of Environment, Zhejiang University of Technology,Hangzhou,China,310014
- Author bio：
- Funds：
- DOI：10.1016/j.cjche.2019.11.012
  CLC：
- Published：2020
- Accepted：
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cellulase cocktail and improvement of its activity[J]. 中国化学工程学报, 2020, 28(4): 1120-1128.
DOI：

cellulase cocktail and improvement of its activity[J]. 中国化学工程学报, 2020, 28(4): 1120-1128. DOI： 10.1016/j.cjche.2019.11.012.

摘要

Abstract

The cellulase cocktail produced by marine

Aspergillus niger

exhibits a property of salt-tolerance

which is of great potential in cellulose degradation in high salt environment. In order to explain the mechanism on the salttolerance of the cellulase cocktail produced by marine

A. niger

six cellulase

components (

Cel6

Cel7A

Cel7B

EGL

BGL1 and

BGL2) were obtained by directed expression. Studies on their enzymatic properties revealed that one β-glucosidase (

BGL2) and one endoglucanase (

EGL) exhibited an outstanding salttolerant property

and one cellobiohydrolase (

Cel7B) exhibited a certain salt-tolerant property. Subsequent study revealed that the salt-tolerant

EGL and

Cel7B endowed the cellulase cocktail with stronger salttolerant property

while the salt-tolerant

BGL2 had no positive effect. Moreover

after overexpression of

Cel6

Cel7A

Cel7B and

EGL

the activity of cellulase cocktail increased by 80%

70%

63% and 68%

respectively. However

the activity of cellulase cocktail was not improved after overexpression of

BGL1 and

BGL2. After mixed-strain fermentation with cellobiohydrolase recombinants (

cel6a

cel7a

and

cel7b

recombinants) and endoglucanase recombinant (egl recombinant)

the the activity of cellulase cocktail increased by 114%

102% and 91%

respectively.

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Salt-tolerant mechanism of marine Aspergillus niger cellulase cocktail and improvement of its activity

DOI：10.1016/j.cjche.2019.11.012

摘要

Abstract

关键词

Keywords

references