Lysozyme adsorption to cation exchanger derivatized by sequential modification of poly(ethylenimine)-Sepharose with succinic anhydride and ethanolamine: Effect of pH and ionic strength

Yangyang Zhao; Xianxiu Li; Linling Yu; Xiaoyan Dong; Yan Sun

doi:10.1016/j.cjche.2019.07.003

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Lysozyme adsorption to cation exchanger derivatized by sequential modification of poly(ethylenimine)-Sepharose with succinic anhydride and ethanolamine: Effect of pH and ionic strength

Separation Science and Engineering | Updated：2026-01-06

- Lysozyme adsorption to cation exchanger derivatized by sequential modification of poly(ethylenimine)-Sepharose with succinic anhydride and ethanolamine: Effect of pH and ionic strength
- Chinese Journal of Chemical Engineering Vol. 28, Issue 2, Pages: 440-444(2020)
- Affiliations：
  
  Department of Biochemical Engineering and Key Laboratory of Systems Bioengineering of the Ministry of Education, School of Chemical Engineering and Technology, Tianjin University,Tianjin,China,300354
- Author bio：
- Funds：
- DOI：10.1016/j.cjche.2019.07.003
  CLC：
- Published：2020
- Accepted：
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Yangyang Zhao, Xianxiu Li, Linling Yu, Xiaoyan Dong, Yan Sun. Lysozyme adsorption to cation exchanger derivatized by sequential modification of poly(ethylenimine)-Sepharose with succinic anhydride and ethanolamine: Effect of pH and ionic strength[J]. Chinese Journal of Chemical Engineering, 2020, 28(2): 440-444.
DOI：

Yangyang Zhao, Xianxiu Li, Linling Yu, Xiaoyan Dong, Yan Sun. Lysozyme adsorption to cation exchanger derivatized by sequential modification of poly(ethylenimine)-Sepharose with succinic anhydride and ethanolamine: Effect of pH and ionic strength[J]. Chinese Journal of Chemical Engineering, 2020, 28(2): 440-444. DOI： 10.1016/j.cjche.2019.07.003.

摘要

Abstract

In our previous work

a series of polyethylenimine (PEI)-derived cation exchangers were synthesized using PEIgrafted resin FF-PEI-L740 (ionic capacity

740 mmol·L

-1

) as the basic resin to study lysozyme adsorption and chromatographic behavior. It was found that the resin with an ionic capacity of 630 mmol·L

-1

(FF-PEI-CR630) possessed high adsorption performance towards lysozyme at 0-100 mmol·L

-1

NaCl. Therefore

in this work

FF-PEI-CR630 was selected to study the influences of pH and ionic strength (IS) on protein adsorption and chromatographic behavior towards lysozyme. The increase of lysozyme adsorption capacity in the pH range of 6 to 10 was observed. However

the uptake rate decreased in the pH range of 6 to 8 and then remained essentially unchanged from pH 8 to pH 10. Increasing IS led to decreased protein adsorption capacity and increased uptake rate in different pH ranges. Besides

FF-PEI-CR630 maintained dynamic binding capacity as high as over 150 mg·ml

-1

at pH 8-10 without NaCl. The research has thus provided insight into the selection of proper pH an

d IS conditions for protein purification by using FF-PEI-CR630.

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