Adsorption equilibria of paeoniflorin and albiflorin on a cyano-silica column (CN column) from the solution of supercritical carbon dioxide (scCO

2

) modified with ethanol were studied. The adsorption capacity at 308.15 K

313.15 K

318.15 K and 323.15 K under pressures corresponding to carbon dioxide/ethanol densities from 0.347 g·cm

-3

to 0.662 g·cm

-3

were determined using the elution by characteristic point method (ECP). The effects of temperature and pressure on the solute loading were investigated. The results showed that the lower the temperature

the higher the adsorption capacity. With the decrease of density of scCO

2

the adsorption capacity strengthens. The maximum adsorption capacity of paeoniflorin (albiflorin) on the CN column was 15.24 mg·ml

-1

(31.14 mg·ml

-1

) in the range of 0-1.84 mg·ml

-1

(0-1.67 mg·ml

-1

) of paeoniflorin (albiflorin) standard solution. The adsorption capacity of albiflorin was twice as much as that of paeoniflorin under the same conditions. Adsorption data of paeoniflorin and albiflorin could be well described by the Langmuir model and Freundlich model. Com

pared with the two model fitting results

the adsorption of paeoniflorin and albiflorin belonged to the monolayer adsorption under conditions of 308.15-323.15 K and 10-17 MPa.