A cold adapt and ethanol tolerant endoglucanase from a marine Bacillus subtilis

Dongsheng Xue; Xuhao Zeng; Chunjie Gong; Dongqiang Lin; Shanjing Yao

doi:10.1016/j.cjche.2018.02.007

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A cold adapt and ethanol tolerant endoglucanase from a marine Bacillus subtilis

Biotechnology and Bioengineering | Updated：2026-01-06

- A cold adapt and ethanol tolerant endoglucanase from a marine Bacillus subtilis
- Chinese Journal of Chemical Engineering Vol. 26, Issue 12, Pages: 2601-2606(2018)
- Affiliations：
  
  2 Department of Chemical and Bioengineering, Zhejiang University,Hangzhou,China,310027
- Author bio：
- Funds：
- DOI：10.1016/j.cjche.2018.02.007
  CLC：
- Published：2018
- Accepted：
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[J]. Chinese Journal of Chemical Engineering, 2018, 26(12): 2601-2606.
DOI：

[J]. Chinese Journal of Chemical Engineering, 2018, 26(12): 2601-2606. DOI： 10.1016/j.cjche.2018.02.007.

摘要

Abstract

The catalytic properties and thermodynamic kinetics of the endoglucanase from a marine Bacillus subtilis were analyzed. Optimum pH and temperature of the endoglucanase activity were 5.0 and 35℃. The endoglucanase activity

melt point temperature was 1.13 folds (247.02 U·ml

-1

)

2.1℃ higher (39.2℃) in 6% ethanol solution than that (218.60 U·ml

-1

)

(37.1℃) in free ethanol. At 40℃-55℃

Gibbs free energy

ΔG

and the content of α-helix was higher in 6% ethanol solution than that in ethanol free solution. The increasing of α-helix content led to higher activity and better thermostability in ethanol solution. The cold adapt ethanol tolerant endoglucanase was valuable for bioethanol product by simultaneous saccharification and fermentation process.

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