Protein adsorption onto diethylaminoethyl dextran modified anion exchanger: Effect of ionic strength and column behavior

Shu Bai; Lingli Gong; Detao Han; Yutong Li; Linling Yu; Yan Sun

doi:10.1016/j.cjche.2017.07.013

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Protein adsorption onto diethylaminoethyl dextran modified anion exchanger: Effect of ionic strength and column behavior

Separation Science and Engineering | Updated：2026-01-06

- Protein adsorption onto diethylaminoethyl dextran modified anion exchanger: Effect of ionic strength and column behavior
- Chinese Journal of Chemical Engineering Vol. 26, Issue 2, Pages: 259-267(2018)
- Affiliations：
  
  Department of Biochemical Engineering and Key Laboratory of Systems Bioengineering of Ministry of Education, School of Chemical Engineering and Technology, Tianjin University,Tianjin,China,300072
- Author bio：
- Funds：
- DOI：10.1016/j.cjche.2017.07.013
  CLC：
- Published：2018
- Accepted：
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Shu Bai, Lingli Gong, Detao Han, Yutong Li, Linling Yu, Yan Sun. Protein adsorption onto diethylaminoethyl dextran modified anion exchanger: Effect of ionic strength and column behavior[J]. Chin.J.Chem.Eng., 2018, 26(2): 259-267.
DOI：

Shu Bai, Lingli Gong, Detao Han, Yutong Li, Linling Yu, Yan Sun. Protein adsorption onto diethylaminoethyl dextran modified anion exchanger: Effect of ionic strength and column behavior[J]. Chin.J.Chem.Eng., 2018, 26(2): 259-267. DOI： 10.1016/j.cjche.2017.07.013.

摘要

Abstract

Our previous studies on bovine serum albumin (BSA) adsorption to diethylaminoethyl dextran (DEAE dextran

DexD

grafting-ligand) and DEAE (D

surface-ligand) modified Sepharose FF resins found that all the grafted resins (FF-DexD and FF-D-DexD) exhibited extremely fast uptake rate (effective diffusivity

N 1.4)

which was six times greater than the ungrafted resins (D

b 0.3). In this work

the influence of ionic strength (IS) on 6 typical DEAE dextran-grafted resins was investigated. Bath adsorption equilibria and kinetics

breakthrough

and linear gradient elution experiments were conducted. Commercial DEAE Sepharose FF was used for comparison. It is found that protein adsorption capacities on DEAE dextran-FF resins and the commercial resin decreased with increasing IS

but DEAE dextran-FF resins exhibited much higher capacity sensitivity to salt concentration. Besides

steeper decrease of adsorption capacities could be obtained at higher graftingligand or surface-ligand density. It is worth noting that the facilitating role of surface-ligand to the "chain delivery" effect was weakened after adding sal

leading to the less improvement in uptake rate by increasing surface-ligand density at higher IS. Although the uptake rates of the DEAE dextran-FF resins increased first and then decreased with increasing IS

they kept the extremely high level of D

values (D

N 1.1) at the their working/binding IS range. Moreover

the DEAE dextran-FF resin displayed much higher adsorption capacities and D

values than commercial ungrafted resin in their working condition. Furthermore

the column results of DEAE dextran-FF resins presented higher dynamic binding capacities than and similar elution ISs with DEAE Sepharose FF to achieve similar (or even higher) recoveries suggest the excellent chromatographic column performance of the DEAE dextran-FF resins. Finally

both high recovery and purity of BSA and γ-globulin could be easily achieved using the typical DEAE dextran-FF column

FF-D60-DexD160

to separate their binary mixtures

by step gradient elution. The research has provided new insights into the practical application of the series of DEAE-dextran grafted resins in protein chromatography and proved their superiority.

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