A strong adhesive block polymer coating for antifouling of large molecular weight protein

Xianling Meng; Xia Jiang; Peijun Ji

doi:10.1016/j.cjche.2017.04.007

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A strong adhesive block polymer coating for antifouling of large molecular weight protein

第25届中国过程控制会议专栏 | Updated：2026-01-06

- A strong adhesive block polymer coating for antifouling of large molecular weight protein
- Chinese Journal of Chemical Engineering Vol. 25, Issue 12, Pages: 1831-1837(2017)
- Affiliations：
  
  Department of Chemical Engineering, Beijing University of Chemical Technology,Beijing,China,100029
- Author bio：
- Funds：
- DOI：10.1016/j.cjche.2017.04.007
  CLC：
- Published：2017
- Accepted：
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Xianling Meng, Xia Jiang, Peijun Ji. A strong adhesive block polymer coating for antifouling of large molecular weight protein[J]. Chin.J.Chem.Eng., 2017, 25(12): 1831-1837.
DOI：

Xianling Meng, Xia Jiang, Peijun Ji. A strong adhesive block polymer coating for antifouling of large molecular weight protein[J]. Chin.J.Chem.Eng., 2017, 25(12): 1831-1837. DOI： 10.1016/j.cjche.2017.04.007.

摘要

Abstract

Some proteins secreted by microorganisms have large molecularweights.Wereport here an approach to prepare coating by multilayer polymers for antifouling of proteins

especially the proteins with a large molecular weight. Stainless steel was used as the model substrate. The substrate was first coatedwith a hybrid polymer film

which was formed by simultaneous hydrolytic polycondensation of 3-aminopropyltriethoxysilane and polymerization of dopamine (HPAPD). After grafting the macroinitiator 2-bromoisobutyryl bromide

the block polymer brushes PMMA-b-PHEMA were grafted. Three proteins were used to test protein adsorption and antifouling behavior of the coating

including recombinant green fluorescent (54 kDa)

recombinant R-transaminase (2×90 kDa)

and recombinant catalase (4×98 kDa). It is demonstrated that the block polymer brushes not only can prevent the adsorption of small molecular weight proteins

but also can significantly reduce the adsorption of the large molecular weight proteins.

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