Combination of a crude oil-degrading bacterial consortium under the guidance of strain tolerance and a pilot-scale degradation test

Yilin Liu; Chen Li; Lei Huang; Yun He; Tingting Zhao; Bo Han; Xiaoqiang Jia

doi:10.1016/j.cjche.2017.02.001

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Combination of a crude oil-degrading bacterial consortium under the guidance of strain tolerance and a pilot-scale degradation test

第25届中国过程控制会议专栏 | Updated：2026-01-06

- Combination of a crude oil-degrading bacterial consortium under the guidance of strain tolerance and a pilot-scale degradation test
- Chinese Journal of Chemical Engineering Vol. 25, Issue 12, Pages: 1838-1846(2017)
- Affiliations：
  
  1. Key Laboratory of Systems Bioengineering (Tianjin University), Ministry of Education,Tianjin,China,300072
  2. Collaborative Innovation Center of Chemical Science and Engineering (Tianjin),Tianjin,China,300072
- Author bio：
- Funds：
- DOI：10.1016/j.cjche.2017.02.001
  CLC：
- Published：2017
- Accepted：
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Yilin Liu, Chen Li, Lei Huang, Yun He, Tingting Zhao, Bo Han, Xiaoqiang Jia. Combination of a crude oil-degrading bacterial consortium under the guidance of strain tolerance and a pilot-scale degradation test[J]. Chin.J.Chem.Eng., 2017, 25(12): 1838-1846.
DOI：

Yilin Liu, Chen Li, Lei Huang, Yun He, Tingting Zhao, Bo Han, Xiaoqiang Jia. Combination of a crude oil-degrading bacterial consortium under the guidance of strain tolerance and a pilot-scale degradation test[J]. Chin.J.Chem.Eng., 2017, 25(12): 1838-1846. DOI： 10.1016/j.cjche.2017.02.001.

摘要

Abstract

Under the guidance of strain tolerance

a new combinationmethod for crude oil-degrading bacterial consortium was studied. Firstly

more than 50 efficient crude oil-degrading and biosurfactant producing bacteriawere isolated from petroleum-contaminated soil andwater in Tianjin Binhai New Area Oilfield

China. Twenty-four of them were selected for further study. These strains were identified as belonging Pseudomonas aeruginosa

Bacillus subtilis

Brevibacillus brevis

Achromobacter sp.

Acinetobacter venetianus

Lysinibacillus macroides

Klebsiella oxytoca

Stenotrophomonas rhizophila

Rhodococcus sp. and Bacillus thuringiensis. A shake-flask degradation test revealed that 12 of these strains could degrade over 50% of 1% crude oil concentration in 7 d. Of these

8 strains were able to produce biosurfactants. Furthermore

environmental tolerance experiments indicated that the majority of the strains had the ability to adapt to extreme environments including high temperatures

alkaline environments and high salinity environments. A mixed bacterial agent comprising the strains WB2

W3 and HA was developed based on the environmental tolerance tests and subjected to the pilot-scale degradation test indicating that this bacterial agent could degrade 85.2% of 0.8% crude oil concentration in 60 d. Our results suggest that the application of this mixed agent could remediate crude oil polluted soils in saline and alkaline environments.

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