Preparation of cross-linked enzyme aggregates of nitrile hydratase ES-NHT-118 from <i>E. coli</i> by macromolecular cross-linking agent

Liya Zhou; Haixia Mou; Jing Gao; Li Ma; Ying He; Yanjun Jiang

doi:10.1016/j.cjche.2016.08.027

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Preparation of cross-linked enzyme aggregates of nitrile hydratase ES-NHT-118 from E. coli by macromolecular cross-linking agent

Biotechnology and Bioengineering | Updated：2026-01-06

- Preparation of cross-linked enzyme aggregates of nitrile hydratase ES-NHT-118 from E. coli by macromolecular cross-linking agent
- Chinese Journal of Chemical Engineering Vol. 25, Issue 4, Pages: 487-492(2017)
- Affiliations：
  
  1. School of Chemical Engineering and Technology, Hebei University of Technology,Tianjin,China,300130
  2. Hebei Provincial Key Lab of Green Chemical Technology and High Efficient Energy Saving, Hebei University of Technology,Tianjin,China,300130
- Author bio：
- Funds：
- DOI：10.1016/j.cjche.2016.08.027
  CLC：
- Published：2017
- Accepted：
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by macromolecular cross-linking agent[J]. , 2017, 25(4): 487-492.
DOI：

by macromolecular cross-linking agent[J]. , 2017, 25(4): 487-492. DOI： 10.1016/j.cjche.2016.08.027.

摘要

Abstract

Cross-linked enzyme aggregates (CLEAs) of nitrile hydratase (NHase) ES-NHT-118 from Escherichia coli were prepared by using ammonium sulfate as precipitating agent followed by cross-linking with dextran polyaldehyde for the first time.In this process

egg white was added as protein feeder for facilitating the formation of CLEAs.The optimal conditions of the immobilization process were determined.Michaelis constants (

) of free NHase and NHase CLEAs were also determined.The NHase CLEAs exhibited increased stability at varied pH and temperature conditions compared to its free counterpart.When exposed to high concentrations of acrylamide

NHase CLEAs also exhibited effective catalytic activity.

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Preparation of cross-linked enzyme aggregates of nitrile hydratase ES-NHT-118 from E. coli by macromolecular cross-linking agent

DOI：10.1016/j.cjche.2016.08.027

摘要

Abstract

关键词

Keywords

references