Improvement of the riboflavin production by engineering the precursor biosynthesis pathways in Escherichia coli

Zhibo Xu; Zhenquan Lin; Zhiwen Wang; Tao Chen

doi:10.1016/j.cjche.2015.08.013

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Improvement of the riboflavin production by engineering the precursor biosynthesis pathways in Escherichia coli

生物技术与生物工程 | Updated：2026-01-06

- Improvement of the riboflavin production by engineering the precursor biosynthesis pathways in Escherichia coli
- Chinese Journal of Chemical Engineering Vol. 23, Issue 11, Pages: 1834-1839(2015)
- Affiliations：
  
  1. 2 SynBio Research Platform, Collaborative Innovation Center of Chemical Science and Engineering (Tianjin), School of Chemical Engineering and Technology, Tianjin University,Tianjin,China,300072
  2. 3 Edinburg-Tianjin Joint Research Centre for Systems Biology and Synthetic Biology, Tianjin University,Tianjin,China,300072
- Author bio：
- Funds：
- DOI：10.1016/j.cjche.2015.08.013
  CLC：
- Published：2015
- Accepted：
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[J]. Chinese Journal of Chemical Engineering, 2015, 23(11): 1834-1839.
DOI：

[J]. Chinese Journal of Chemical Engineering, 2015, 23(11): 1834-1839. DOI： 10.1016/j.cjche.2015.08.013.

摘要

Abstract

4-Dihydroxy-2-butanone 4-phosphate (DHBP) and GTP are the precursors for riboflavin biosynthesis. In this research

improving the precursor supply for riboflavin production was attempted by overexpressing ribB and engineering purine pathway in a riboflavin-producing Escherichia coli strain. Initially

ribB gene was overexpressed to increase the flux from ribulose 5-phosphate (Ru-5-P) to DHBP. Then ndk and gmk genes were overexpressed to enhance GTP supply. Subsequently

a R419Lmutationwas introduced into purA to reduce the flux from IMP to AMP. Finally

co-overexpression of mutant purF and prs genes further increased riboflavin production. The final strain RF18S produced 387.6 mg riboflavin·L

-1

with a yield of 44.8 mg riboflavin per gram glucose in shake-flask fermentations. The final titer and yield were 72.2% and 55.6% higher than those of RF01S

respectively. It was concluded that simultaneously engineering the DHBP synthase and GTP biosynthetic pathway by rational metabolic engineering can efficiently boost riboflavin production in E. coli.

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Related Institution

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School of Chemistry and Chemical Engineering, North University of China

2 Department of Chemical Engineering, Xi'an Jiaotong University

3 Department of Environmental and Chemical Engineering, Xi'an Polytechnic University

School of Chemical Engineering, Northwest University

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