Antifouling coatings are used extensively on vessels and underwater structures. Conventional antifouling coatings contain toxic biocides and heavy metals

which may induce unwanted adverse effects such as toxicity to non-target organisms

imposex in gastropods and increased multiresistance among bacteria. Therefore

enzyme-based coatings could be a new alternative solution. A H

2

O

2

-producing bienzyme systemwas developed in this study. H

2

O

2

can be produced from starch by the cooperation of α-amylase and glucose oxidase

which promotes the hydrolysis of polymeric chain and oxidizes the glucose to produce H

2

O

2

respectively. The encapsulated bienzyme (A-G@BS) exhibits enhanced stabilities of thermal

pH

recycling and tolerance of xylene. The A-G@BScontaining coating releases H

2

O

2

at rates exceeding a target of 36 nmol·cm

-2

·d

-1

for 90 days in a laboratory assay. The results demonstrate that the method is a promising coating technology for entrapping active enzymes

presenting an interesting avenue for enzyme-based antifouling solutions.