Fermentation of bioflocculant with Corynebacterium glutamicum was studied by way of kinetic modeling. Lorentzian modified Logistic model

time-corrected Luedeking-Piret and Luedeking-Piret type modelswere proposed and applied to describe the cell growth

bioflocculant synthesis and consumption of substrates

with the correlation of initial biomass concentration and initial glucose concentration

respectively. The results showed that thesemodels could well characterize the batch culture process of C. glutamicumat various initial glucose concentrations from 10.0 to 17.5 g·L

-1

. The initial biomass concentration could shorten the lag time of cell growth

while the maximum biomass concentration was achieved only at the optimal initial glucose concentration of 16.22 g·L

-1

. A novel three-stage fed-batch strategy for bioflocculant production was developed based on the model prediction

in which the lag phase

quick biomass growth and bioflocculant production stages were sequentially proceeded with the adjustment of glucose concentration and dissolved oxygen. Biomass of 2.23 g·L

-1

was obtained and bioflocculant concentration was enhanced to 176.32 mg·L

-1

18.62% and 403.63% higher than those in the batch proces

s

respectively

indicating an efficient fed-batch culture strategy for bioflocculant production.