Production of paeoniflorin and albiflorin by callus tissue culture of <i>Paeonia lactiflora</i> Pall

Sheng Hu; Yingying Ma; Hailong Jiang; Dingjun Feng; Wei Yu; Dongmei Dai; Lehe Mei

doi:10.1016/j.cjche.2014.06.036

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Production of paeoniflorin and albiflorin by callus tissue culture of Paeonia lactiflora Pall

研究简报 | Updated：2026-01-06

- Production of paeoniflorin and albiflorin by callus tissue culture of Paeonia lactiflora Pall
- Chinese Journal of Chemical Engineering Vol. 23, Issue 2, Pages: 451-455(2015)
- Affiliations：
  
  1. College of Biological and Chemical Engineering, Ningbo Institute of Technology, Zhejiang University,Ningbo,China,315100
  2. Department of Chemical and Biochemical Engineering, Zhejiang University,Hangzhou,China,310027
  3. Ningbo Liwah Pharmaceutical Co., Ltd.,Ningbo,China,315174
- Author bio：
- Funds：
- DOI：10.1016/j.cjche.2014.06.036
  CLC：
- Published：2015
- Accepted：
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Pall[J]. , 2015, 23(2): 451-455.
DOI：

Pall[J]. , 2015, 23(2): 451-455. DOI： 10.1016/j.cjche.2014.06.036.

摘要

Abstract

In order to facilitate the preparation of paeoniflorin (PF) and albiflorin (AF)

two chief bioactive constituents in

Paeonia lactiflora

Pall (PL)

induction and culture of callus from PL were studied. With a modified woody plant medium supplemented with 0.5 mg·L

-1

6-benzylaminopurine

1.0 mg·L

-1

naphthylacetic acid

0.1 mg·L

-1

thidiazuron and 30 g·L

-1

sucrose

callus was induced from four kinds of explants: leaf

stems

petiole

and root. The potency to formcallus varies between different explants and leaf explants exhibits the highest capacity (100%). On the other hand

root-derived callus (R-callus) produces the highest level of total amount of PF and AF

31.8 mg·g

-1

dry mass

which is higher than the corresponding level in the root of field cultivated PL. Furthermore

the timeneeded is only 40 days

remarkably shorter than the cultivation timeof PL

about 4-5years. Higher accumulation levels of PF and AF with shorter production time indicate that callus culture of PL is a promising powerful tool for production of PF and AF in the future.

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⁰

Production of paeoniflorin and albiflorin by callus tissue culture of Paeonia lactiflora Pall

DOI：10.1016/j.cjche.2014.06.036

摘要

Abstract

关键词

Keywords

references