Production of Poly(3-hydroxybutyrate-co-3-hydroxyhexanoate) by Recombinant Pseudomonas stutzeri 1317 from Unrelated Carbon Sources

魏晓星; 刘峰; 简嘉; 王瑞妍; 陈国强

doi:10.1016/S1004-9541(13)60549-2

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Production of Poly(3-hydroxybutyrate-co-3-hydroxyhexanoate) by Recombinant Pseudomonas stutzeri 1317 from Unrelated Carbon Sources

生物技术与生物工程 | Updated：2026-01-06

- Production of Poly(3-hydroxybutyrate-co-3-hydroxyhexanoate) by Recombinant Pseudomonas stutzeri 1317 from Unrelated Carbon Sources
- Chinese Journal of Chemical Engineering Vol. 21, Issue 9, Pages: 1057-10611004-954160549-2(2013)
- Affiliations：
  
  1. 2 MOE Key Lab of Bioinformatics and Systems Biology, Department of Biological Science and Biotechnology, School of Life Sciences, Tsinghua-Peking Center for Life Sciences, Tsinghua University,Beijing,China,100084
  2. 3 Tuspark Kunshan Biotech Science Park Development Center, 4/F, Science & Technology Plaza, Tsinghua Science Park, Kunshan,Jiangsu,China,215347
  3. 4 Center for Nano and Micro Mechanics, Tsinghua University,Beijing,China,100084
- Author bio：
- Funds：
- DOI：10.1016/S1004-9541(13)60549-2
  CLC：
- Published：2013
- Accepted：
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1317 from Unrelated Carbon Sources[J]. Chinese Journal of Chemical Engineering, 2013, 21(9): 1057-1061.
DOI：

1317 from Unrelated Carbon Sources[J]. Chinese Journal of Chemical Engineering, 2013, 21(9): 1057-1061. DOI： 10.1016/S1004-9541(13)60549-2.

摘要

Abstract

Synthetic biology promises to simplify the construction of metabolic pathways by assembling the detached modules of the whole pathway. This gives new approaches for the microbial production of industrial products such as polyhydroxyalkanoates (PHA). In this study

to produce poly(3-hydroxybutyrate-co-3-hydroxyhexanoate) (PHBHHx) by Pseudomonas stutzeri 1317 from unrelated carbon sources such as glucose

the phaC1-phaZ-phaC2 operon of P. stutzeri 1317 was knocked out to generate the PHA deficient mutant P. stutzeri 1317LF. Then three modules containing phaC

phaC

and phaC

were introduced into P. stutzeri 1317LF separately. The shake flask results indicated that the precursor supply and PHA synthase activity were the vital factors for the PHBHHx accumulation of P. stutzeri 1317LF. Furthermore

the PHBHHx accumulation of the recombinants from different carbon resources were performed. The highest PHBHHx content was 23.7%(by mass) with 58.6%(by mole) 3HB fraction. These results provide basis for further improving the PHBHHx accumulation of P. stutzeri from unrelated carbon sources.

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