Immobilization of Papain in Biosilica Matrix and Its Catalytic Property

ZHOU Liya; WANG Cui; JIANG Yanjun; GAO Jing

doi:10.1016/S1004-9541(13)60528-5

Your Location：

Home >

Browse articles >

Immobilization of Papain in Biosilica Matrix and Its Catalytic Property

生物技术与生物工程 | Updated：2026-01-06

- Immobilization of Papain in Biosilica Matrix and Its Catalytic Property
- Chinese Journal of Chemical Engineering Issue 6, Pages: 670-6751004-954160528-5(2013)
- Affiliations：
  
  1. School of Chemical Engineering and Technology, Hebei University of Technology,Tianjin,China,300130
  2. 2 National Key Laboratory of Biochemical Engineering, Institute of Process Engineering, Chinese Academy of Sciences,Beijing,China,100190
- Author bio：
- Funds：
- DOI：10.1016/S1004-9541(13)60528-5
  CLC：
- Published：2013
- Accepted：
Scan QR Code
ZHOU Liya, WANG Cui, JIANG Yanjun, et al. Immobilization of Papain in Biosilica Matrix and Its Catalytic Property[J]. Chinese Journal of Chemical Engineering, 2013, (6): 670-6751004-954160528-5.
DOI：

ZHOU Liya, WANG Cui, JIANG Yanjun, et al. Immobilization of Papain in Biosilica Matrix and Its Catalytic Property[J]. Chinese Journal of Chemical Engineering, 2013, (6): 670-6751004-954160528-5. DOI： 10.1016/S1004-9541(13)60528-5.

摘要

Abstract

A novel method was developed for papain immobilization through a biomimetic silicification process induced by papain. By incubating papain in a silica precursor solution

the papain-silica composite formed rapidly and papain was encapsulated. The encapsulation efficiency and the recovery activity were 82.60% and 83.09%

respectively. Compared with enzymes and biomolecules immobilized in biosilica matrix in the presence of additional silica-precipitating species

this papain encapsulation process

a biomimetic approach

realized high encapsulation efficiency by its autosilification activity under mild conditions (near-neutral pH and ambient temperature). Furthermore

the encapsulated papain exhibits enhanced thermal

recycling and storage stabilities. Kinetic analysis showed that the biomimetic silica matrix did not significantly hinder the mass transport of substrate or the release of product.

关键词

Keywords

references

Views

Downloads

CSCD

Alert me when the article has been cited

Submit

Tools

Publicity Resources

Silicalite-1 zeolite encapsulated Fe nanocatalyst for Fenton-like degradation of methylene blue

Concepts, processing, and recent developments in encapsulating essential oils

Supercritical Antisolvent-based Technology for Preparation of Vitamin D3 Proliposome and Its Characteristics

Ag-induced Efficient Immobilization of Papain on Silica Spheres

Preparation of Well-shaped Microcapsule Immobilizing Inorganic Nanoparticles

Related Author

Yan Shao

Huanhao Chen

Xueying Zhang

Linyuan Chen

Hongwei Guo

Guoping Lian

Guanghui Ma

Qiong Cai

Related Institution

State Key Laboratory of Materials-Oriented Chemical Engineering, College of Chemical Engineering, Nanjing Tech University

School of Environmental Science and Engineering, Nanjing Tech University

Department of Chemical and Process Engineering, University of Surrey, Guildford

State Key Laboratory of Biochemical Engineering, Institute of Process Engineering, Chinese Academy of Sciences

University of Chinese Academy of Sciences, School of Chemical Sciences

⁰