Immobilization of Penicillin G Acylase on Magnetic Nanoparticles Modified by Ionic Liquids

周华从; 李伟; 寿庆辉; 高红帅; 徐芃; 邓伏礼; 刘会洲

doi:10.1016/S1004-9541(12)60374-7

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Immobilization of Penicillin G Acylase on Magnetic Nanoparticles Modified by Ionic Liquids

Updated：2026-01-06

- Immobilization of Penicillin G Acylase on Magnetic Nanoparticles Modified by Ionic Liquids
- Chinese Journal of Chemical Engineering Vol. 20, Issue 1, Pages: 146-151(2012)
- Affiliations：
  
  1. State Key Laboratory of Biochemical Engineering, Laboratory of Green Process and Engineering, Institute of Process Engineering, Chinese Academy of Sciences,Beijing,China,100190
  2. Graduate University of Chinese Academy of Sciences,Beijing,China,100049
- Author bio：
- Funds：
- DOI：10.1016/S1004-9541(12)60374-7
  CLC： TQ925
- Published：2012
- Accepted：
Scan QR Code
ZHOU Huacong, LI Wei, SHOU Qinghui, GAO Hongshuai, XU Peng, DENG Fuli, LIU Huizhou. Immobilization of Penicillin G Acylase on Magnetic Nanoparticles Modified by Ionic Liquids[J]. Chin.J.Chem.Eng., 2012, 20(1): 146-151.
DOI：

ZHOU Huacong, LI Wei, SHOU Qinghui, GAO Hongshuai, XU Peng, DENG Fuli, LIU Huizhou. Immobilization of Penicillin G Acylase on Magnetic Nanoparticles Modified by Ionic Liquids[J]. Chin.J.Chem.Eng., 2012, 20(1): 146-151. DOI： 10.1016/S1004-9541(12)60374-7.

摘要

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Abstract

Functionalized ionic liquids containing ethyoxyl groups were synthesized and immobilized on magnetic silica nanoparticles(MSNP) prepared by two steps

i.e.

synthesis and silica shell growth on the surface.This magnetic nanoparticle supported ionic liquid(MNP-IL) were applied in the immobilization of penicillin G acylase(PGA).The MSNPs and MNP-ILs were characterized by the means of Fourier transform infrared spectroscopy(FTIR)

scanning electron microscopy(SEM)

transmission electron microscopy(TEM)

and vibrating sample magnetometer(VSM).The results showed that the average size of magnetic Fe

nanoparticles and MSNPs were ~10 and ~90 nm

respectively.The saturation magnetizations of magnetic Fe

nanoparticles and MNP-ILs were 63.7 and 26.9 A·m

·kg

-1

respectively.The MNP-IL was successfully applied in the immobilization of PGA.The maximum amount of loaded enzyme was about 209 mg·g

-1

(based on carrier)

and the highest enzyme activity of immobilized PGA(based on ImPGA) was 261 U·g

-1

.Both the amount of loaded enzyme and the activity of ImPGA are at the same level of or higher than that in previous reports.After 10 consecutive operations

ImPGA still maintained 62% of its initial activity

indicating the good recovery property of ImPGA activity.The ionic liqui

d modified magnetic particles integrate the magnetic properties of Fe

and the structure-tunable properties of ionic liquids

and have extensive potential uses in protein immobilization and magnetic bioseparation.This work may open up a novel strategy to immobilize proteins by ionic liquids.

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