Biodesulfurization(BDS) is a promising technology for deep desulfurization.In this work
Pseudomonas delafieldii
R-8 cells are immobilized in calcium alginate beads and used for BDS of transportation fuels.It is found that thiophene and dibenzothiophene(DBT) can be simultaneously metabolized by immobilized R-8 cells.The initial sulfur content in the model oil is 300 mg·kg
-1
(thiophene:DBT 1:1).After 10 h of treatment
the thiophene concentration is reduced by 40%
while DBT is reduced by 25%.The utilization rate of thiophene is faster than that of DBT.Moreover
the oil/water ratio of alginate immobilized cells is studied to reduce the water volume in desulfurization systems.Long-term recycling of BDS by alginate immobilized cells is carried out with oil/water ratio at 5:1.The immobilized cells are successfully reused over 15 batch cycles.In the last batch
the desulfurization activity remains at least 75% of the first batch.
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