Isolation of Lysozyme from Chicken Egg White Using Polyacrylamide-based Cation-exchange Cryogel

晏禄丁; 沈绍传; 贠军贤; 姚克俭

doi:10.1016/S1004-9541(11)60068-2

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Isolation of Lysozyme from Chicken Egg White Using Polyacrylamide-based Cation-exchange Cryogel

Updated：2026-01-06

- Isolation of Lysozyme from Chicken Egg White Using Polyacrylamide-based Cation-exchange Cryogel
- Chinese Journal of Chemical Engineering Vol. 19, Issue 5, Pages: 876-880(2011)
- Affiliations：
  
  State Key Laboratory Breeding Base of Green Chemistry Synthesis Technology, College of Chemical Engineering and Materials Science, Zhejiang University of Technology,Hangzhou,China,310032
- Author bio：
- Funds：
- DOI：10.1016/S1004-9541(11)60068-2
  CLC：
- Published：2011
- Accepted：
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YAN Luding, SHEN Shaochuan, YUN Junxian, YAO Kejian. Isolation of Lysozyme from Chicken Egg White Using Polyacrylamide-based Cation-exchange Cryogel[J]. , 2011, 19(5): 876-880.
DOI：

YAN Luding, SHEN Shaochuan, YUN Junxian, YAO Kejian. Isolation of Lysozyme from Chicken Egg White Using Polyacrylamide-based Cation-exchange Cryogel[J]. , 2011, 19(5): 876-880. DOI： 10.1016/S1004-9541(11)60068-2.

摘要

Abstract

An effective cation-exchange chromatographic method for lysozyme isolation from chicken egg white is presented

using supermacroporous cryogel grafted with sulfo functional groups. The chromatographic processes were carried out by one-step and sequential elution

respectively. Sodium phosphate buffer (pH 7.8) containing different concentrations of NaCl is used as elution agent. The corresponding breakthrough characteristics and elution behaviors in the cryogel bed were investigated and analyzed. Purity of lysozyme in the elution effluent was assayed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE). The maximum purity of the obtained lysozyme was about 96%

and the cryogel is demonstrated as a potential separation medium for purification of high-purity lysozyme from chicken egg white.

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