Enzymatic Hydrolysis of Cellulose with Different Crystallinities Studied by Means of SEC-MALLS

张名佳; 苏荣欣; 齐崴; 杜若愚; 何志敏

doi:10.1016/S1004-9541(11)60055-4

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Enzymatic Hydrolysis of Cellulose with Different Crystallinities Studied by Means of SEC-MALLS

Updated：2026-01-06

- Enzymatic Hydrolysis of Cellulose with Different Crystallinities Studied by Means of SEC-MALLS
- Chinese Journal of Chemical Engineering Vol. 19, Issue 5, Pages: 773-778(2011)
- Affiliations：
  
  State Key Laboratory of Chemical Engineering, Chemical Engineering Research Center, School of Chemical Engi-neering and Technology, Tianjin University,Tianjin,China,300072
- Author bio：
- Funds：
- DOI：10.1016/S1004-9541(11)60055-4
  CLC：
- Published：2011
- Accepted：
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ZHANG Mingjia, SU Rongxin, QI Wei, DU Ruoyu, HE Zhimin. Enzymatic Hydrolysis of Cellulose with Different Crystallinities Studied by Means of SEC-MALLS[J]. , 2011, 19(5): 773-778.
DOI：

ZHANG Mingjia, SU Rongxin, QI Wei, DU Ruoyu, HE Zhimin. Enzymatic Hydrolysis of Cellulose with Different Crystallinities Studied by Means of SEC-MALLS[J]. , 2011, 19(5): 773-778. DOI： 10.1016/S1004-9541(11)60055-4.

摘要

Abstract

The reactions of exo-cellulase (cellobiohydrolase

CBH) and endo-cellulase (endoglucanase

EG) were investigated by analyzing the insoluble residues of microcrystalline cellulose (MCC) and filter paper cellulose (FPC) during enzymatic hydrolysis. Molecular parameters including molecular weight and its distribution

degree of po-lymerization

and radii of gyration were measured by size exclusion chromatography coupled with multi-angle laser light scattering. No significant change in MCC chains was found during the whole reaction period

indicating that CBH digestion follows a layer-by-layer solubilization manner. This reaction mode might be the major reason for slow enzymatic hydrolysis of cellulose. On the other hand

the degree of polymerization of FPC chains decreases rapidly in the initial reaction

indicating that EG digestion follows a random scission manner

which may create new ends for CBH easily. The slopes of the conformation plots for MCC and FPC increase gradually

indicating stronger chain stiffness of cellulose during hydrolysis.

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