Biocontrol Efficiency of <i>Bacillus subtilis</i> SL-13 and Characterization of an Antifungal Chitinase

刘燕; 陶晶; 阎豫君; 李彬; 李晖; 李春

doi:10.1016/S1004-9541(09)60188-9

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Biocontrol Efficiency of Bacillus subtilis SL-13 and Characterization of an Antifungal Chitinase

Updated：2026-01-06

- Biocontrol Efficiency of Bacillus subtilis SL-13 and Characterization of an Antifungal Chitinase
- Chinese Journal of Chemical Engineering Vol. 19, Issue 1, Pages: 128-134(2011)
- Affiliations：
  
  1. School of Life Science&Technology, Beijing Institute of Technology,Beijing,China,100081
  2. School of Agriculture/Key Laboratory for Green Processing of Chemical Engineering of Xinjiang Bingtuan, Shihezi University,Xinjiang,China,832003
- Author bio：
- Funds：
- DOI：10.1016/S1004-9541(09)60188-9
  CLC：
- Published：2011
- Accepted：
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SL-13 and Characterization of an Antifungal Chitinase[J]. , 2011, 19(1): 128-134.
DOI：

SL-13 and Characterization of an Antifungal Chitinase[J]. , 2011, 19(1): 128-134. DOI： 10.1016/S1004-9541(09)60188-9.

摘要

Abstract

The seed germination and tomato seedling tests showed that

Bacillus subtilis

SL-13 could promote the sprouting and seedling growth of tomato.The fresh and dry weight of tomato seedlings increased 42.86%and 18.75%

respectively.The control efficacies of the SL-13 to tomato

Rhizoctonia

rot were 20.65% and 35.23% in the greenhouse and field

respectively.The growth of the plant-pathogenic fungus Rhizoctonia solani was considerably inhibited in the presence of the strain SL-13 culture supernatant.The main antifungal protein was detected to be chitinase through vitro assay.The chitinase was purified with DEAE-Sepharose fast flow ion exchange column chromatography and Sephadex G-75 gel filtration for further characterization.The optimal pH and temperature for the chitinase activity were 7.0 and 50℃

respectively.It was demonstrated that the enzyme was stable at pH 5-9 and 40-60℃.70% of the enzyme activity was retained when incubated at 121℃ and 0.11 MPa for 20 min

and the enzyme was not sensitive to protease K and ultraviolet radiation.Thus it is suitable for effective biological control in relatively unstable environment.

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Biocontrol Efficiency of Bacillus subtilis SL-13 and Characterization of an Antifungal Chitinase

DOI：10.1016/S1004-9541(09)60188-9

摘要

Abstract

关键词

Keywords

references