Fluorescent Proteins as a Visible Molecular Signal for Rapid Quantification of Bioprocesses:Potential and Challenges

张翀; 邢新会

doi:10.1016/S1004-9541(09)60140-3

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Fluorescent Proteins as a Visible Molecular Signal for Rapid Quantification of Bioprocesses:Potential and Challenges

Updated：2026-01-06

- Fluorescent Proteins as a Visible Molecular Signal for Rapid Quantification of Bioprocesses:Potential and Challenges
- Chinese Journal of Chemical Engineering Vol. 18, Issue 5, Pages: 863-869(2010)
- Affiliations：
  
  Department of Chemical Engineering, Tsinghua University,Beijing,China,100084
- Author bio：
- Funds：
- DOI：10.1016/S1004-9541(09)60140-3
  CLC：
- Published：2010
- Accepted：
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ZHANG Chong, XING Xinhui. Fluorescent Proteins as a Visible Molecular Signal for Rapid Quantification of Bioprocesses:Potential and Challenges[J]. , 2010, 18(5): 863-869.
DOI：

ZHANG Chong, XING Xinhui. Fluorescent Proteins as a Visible Molecular Signal for Rapid Quantification of Bioprocesses:Potential and Challenges[J]. , 2010, 18(5): 863-869. DOI： 10.1016/S1004-9541(09)60140-3.

摘要

Abstract

Green fluorescent protein (GFP) and its variants/homolog proteins are generally called as GFP-like fluorescent proteins (FPs)

which are widely used as visible molecular tools for monitoring a wide range of biological processes due to their capability of simple

accurate and real time quantification. The FPs-based molecular and visible quantification tools are giving more impact on bioprocess engineering

enabling the biomolecule-level dynamic information to be linked with the process-level events. In this review

different applications of FPs in biological engineering with emphasis on rapid molecular bioprocess quantification

such as quantification of the transcription efficiency

the protein production

the protein folding efficiency

the cell concentration

the intracellular microenvironments and so on

would be first introduced. The challenges of using FPs with respect to actual bioprocess applications for the precise quantification including the interaction of FPs and the fused partner proteins

the maturation of FPs

the inner filter effect and sensing technology were then discussed. Finally

the future development for the FPs used in molecular bioprocess quantification would be proposed.

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